Phylogenetic landscape of Monkeypox Virus (MPV) during the early outbreak in New York City, 2022.
Phylogenetic landscape of Monkeypox Virus (MPV) during the early outbreak in New York City, 2022.
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DOI：
10.1080/22221751.2023.2192830
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发表时间：
2023-12
影响因子：
13.2
通讯作者：
中科院分区：
文献类型：
--
作者：
猴痘（MFOX）是一种流行于非洲中部/西部地区的人畜共患疾病。MPV的地理地方性已经扩大，扩大了人猴痘病毒的界面及其溢出的可能性。自2022年5月以来，一场没有被证实与流行国家有联系的多国MPV大规模疫情起源于欧洲，并迅速在地球仪各地蔓延，引发了基因组监测工作。在此，我们对早期暴发期间来自纽约市的23名MPV感染患者进行了基因组分析，评估了这些毒株与公开的MPV基因组的系统发育关系。此外，我们比较了临床分离株与来自样本子集的培养传代样本的基因组序列。系统发育分析显示，本研究中包括的MPV基因组在B.1谱系（进化枝IIb）内聚类，其中一些样品进一步分化为B.1的五个不同亚谱系。突变分析揭示了整个基因组中的55个非同义多态性，其中一些突变位于病毒增殖、结构和组装功能所需的关键区域，以及抗病毒治疗的靶区域。此外，我们鉴定了与GA > AA和TC > TT核苷酸替换相关的大多数多态性，表明人APOBEC 3酶的作用。临床分离株和细胞培养传代样本之间的比较未能显示任何差异。我们的研究结果提供了对纽约市早期MPV-2022（B.1）循环菌株突变景观的初步了解。
Monkeypox (MPOX) is a zoonotic disease endemic to regions of Central/Western Africa. The geographic endemicity of MPV has expanded, broadening the human-monkeypox virus interface and its potential for spillover. Since May 2022, a large multi-country MPV outbreak with no proven links to endemic countries has originated in Europe and has rapidly expanded around the globe, setting off genomic surveillance efforts. Here, we conducted a genomic analysis of 23 MPV-infected patients from New York City during the early outbreak, assessing the phylogenetic relationship of these strains against publicly available MPV genomes. Additionally, we compared the genomic sequences of clinical isolates versus culture-passaged samples from a subset of samples. Phylogenetic analysis revealed that MPV genomes included in this study cluster within the B.1 lineage (Clade IIb), with some of the samples displaying further differentiation into five different sub-lineages of B.1. Mutational analysis revealed 55 non-synonymous polymorphisms throughout the genome, with some of these mutations located in critical regions required for viral multiplication, structural and assembly functions, as well as the target region for antiviral treatment. In addition, we identified a large majority of polymorphisms associated with GA > AA and TC > TT nucleotide replacements, suggesting the action of human APOBEC3 enzyme. A comparison between clinical isolates and cell culture-passaged samples failed to reveal any difference. Our results provide a first glance at the mutational landscape of early MPV-2022 (B.1) circulating strains in NYC.
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影响因子：
82.9
作者：
Isidro, Joana;Borges, Vitor;Pinto, Miguel;Sobral, Daniel;Santos, Joao Dourado;Nunes, Alexandra;Mixao, Veronica;Ferreira, Rita;Santos, Daniela;Duarte, Silvia;Vieira, Luis;Borrego, Maria Jose;Nuncio, Sofia;de Carvalho, Isabel Lopes;Pelerito, Ana;Cordeiro, Rita;Gomes, Joao Paulo
通讯作者：
Gomes, Joao Paulo
影响因子：
4.4
作者：
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通讯作者：
Zumla, Alimuddin
影响因子：
0.8
作者：
Cohen-Gihon, Inbar;Israeli, Ofir;Zvi, Anat
通讯作者：
Zvi, Anat
2001年至2018年之间，中非共和国的人类猴痘的基因组史。
影响因子：
4.6
作者：
Berthet N;Descorps-Declère S;Besombes C;Curaudeau M;Nkili Meyong AA;Selekon B;Labouba I;Gonofio EC;Ouilibona RS;Simo Tchetgna HD;Feher M;Fontanet A;Kazanji M;Manuguerra JC;Hassanin A;Gessain A;Nakoune E
通讯作者：
Nakoune E
影响因子：
10.7
作者：
Firth C;Kitchen A;Shapiro B;Suchard MA;Holmes EC;Rambaut A
通讯作者：
Rambaut A