Subsets of myeloid-derived suppressor cells in tumor-bearing mice.
Subsets of myeloid-derived suppressor cells in tumor-bearing mice.
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骨髓抑制细胞的子集中肿瘤小鼠的子集。
DOI：
10.4049/jimmunol.181.8.5791
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发表时间：
2008-10-15
影响因子：
4.4
通讯作者：
Gabrilovich, Dmitry I.
中科院分区：
文献类型：
--
作者：
Youn, Je-In;Nagaraj, Srinivas;Collazo, Michelle;Gabrilovich, Dmitry I.
髓源性抑制细胞（MDSC）是一组异质性细胞，在肿瘤相关的免疫抑制中起关键作用。为了确定MDSC中对这些细胞的免疫抑制特性起主要作用的特定亚群，对10种不同的肿瘤模型进行了研究。所有模型都显示MDSC的数量有不同程度但显著的增加。体内MDSC扩增的变异性与肿瘤细胞条件培养基（TCCM）在体外的作用密切匹配。MDSC由Ly6G⁺Ly6C⁽低⁾粒细胞和Ly6G⁻Ly6C⁽高⁾单核细胞这两个主要亚群组成。粒细胞性MDSC的活性氧物质（ROS）水平升高，一氧化氮（NO）水平检测不到，而单核细胞性MDSC的NO水平升高，但ROS水平检测不到。然而，它们单个细胞的抑制活性是相当的。几乎所有肿瘤模型都显示粒细胞性MDSC亚群优先扩增。我们对之前被认为参与MDSC介导的T细胞抑制的几种表面分子进行了表型和功能分析：CD115、CD124、CD80、PD - L1和PD - L2。尽管相当比例的MDSC表达这些分子，但在MDSC与来自无免疫抑制活性的无肿瘤小鼠的细胞之间，未发现它们的表达水平或阳性细胞比例有差异。MDSC介导的T细胞抑制水平并不取决于这些分子的表达。这些数据表明，MDSC的抑制特性不是由特定亚群的扩增引起的，而更可能代表这些细胞的一种功能状态。
Myeloid-derived suppressor cells (MDSC) are a heterogeneous group of cells that play a critical role in tumor associated immune suppression. In an attempt to identify a specific subset of MDSC primarily responsible for immunosuppressive features of these cells, 10 different tumor models were investigated. All models showed variable but significant increase in the population of MDSC. Variability of MDSC expansion in vivo matched closely the effect of tumor-cell condition media (TCCM) in vitro. MDSC consists of two major subsets of Ly6G+Ly6Clow granulocytic and Ly6G-Ly6Chigh monocytic cells. Granulocytic MDSC have increased level of reactive oxygen species (ROS) and undetectable level of nitric oxide (NO) whereas monocytic MDSC had increased level of NO but undetectable levels of ROS. However, their suppressive activity per cell basis was comparable. Almost all tumor models demonstrated a preferential expansion of granulocytic subset of MDSC. We performed a phenotypical and functional analysis of several surface molecules previously suggested to be involved in MDSC mediated suppression of T cells: CD115, CD124, CD80, PD-L1, and PD-L2. Although substantial proportion of MDSC expressed those molecules no differences in the level of their expression or the proportion, positive cells were found between MDSC and cells from tumor-free mice that lack immune suppressive activity. The level of MDSC mediated T-cell suppression did not depend on the expression of these molecules. This data indicates that suppressive features of MDSC is caused not by expansion of a specific subset but more likely represents a functional state of these cells.
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免疫调节配体的B7家族。
影响因子：
12.3
作者：
Collins, Mary;Ling, Vincent;Carreno, Beatriz M
通讯作者：
Carreno, Beatriz M
影响因子：
4.4
作者：
Almand, B;Clark, JI;Gabrilovich, DI
通讯作者：
Gabrilovich, DI
影响因子：
4.4
作者：
Atochina, O;Daly-Engel, T;Harn, DA
通讯作者：
Harn, DA
DOI：
10.1084/jem.20062602
发表时间：
2007-06-11
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Delano MJ;Scumpia PO;Weinstein JS;Coco D;Nagaraj S;Kelly-Scumpia KM;O'Malley KA;Wynn JL;Antonenko S;Al-Quran SZ;Swan R;Chung CS;Atkinson MA;Ramphal R;Gabrilovich DI;Reeves WH;Ayala A;Phillips J;Laface D;Heyworth PG;Clare-Salzler M;Moldawer LL
通讯作者：
Moldawer LL
DOI：
10.1084/jem.20050930
发表时间：
2006-04-17
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Kryczek I;Zou L;Rodriguez P;Zhu G;Wei S;Mottram P;Brumlik M;Cheng P;Curiel T;Myers L;Lackner A;Alvarez X;Ochoa A;Chen L;Zou W
通讯作者：
Zou W