Evaluation of Effects of TGF-β1 Inhibition on Gastric Cancer in Nude Mice by Diffusion Kurtosis Imaging and In-Line X-ray Phase Contrast Imaging With Sequential Histology.
Evaluation of Effects of TGF-β1 Inhibition on Gastric Cancer in Nude Mice by Diffusion Kurtosis Imaging and In-Line X-ray Phase Contrast Imaging With Sequential Histology.
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通过扩散峰度成像和串联 X 射线相差成像与序贯组织学评价 TGF-β 1 抑制对裸鼠胃癌的影响
DOI：
10.1002/jmri.26523
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发表时间：
2019-06
期刊：
影响因子：
--
通讯作者：
Du L
中科院分区：
文献类型：
--
作者：
Shi B;Yuan F;Yan F;Zhang H;Pan Z;Chen W;Wang G;Tan J;Zhang Y;Ren Y;Du L
治疗后准确、完整的疗效评价对实施胃癌个体化治疗具有重要意义。研究扩散峰度成像（DKI）和在线X线相位对比成像（ILXPCI）在通过抑制转化生长因子β 1（TGF-β1）评估治疗疗效中的有效性。前瞻性动物研究。30只裸鼠胃癌皮下异种移植瘤模型用于DKI，10只裸鼠腹膜转移瘤模型用于ILXPCI。在TGF-β1抑制治疗前和治疗后7、14、21和28天连续进行检查，包括T2加权成像（T2 WI）和DKI，5个B值为0、500、1000、1500、2000 s/mm 2;在治疗后14天进行ILXPCI检查。DKI参数（表观扩散系数[ADC]、扩散系数[D]和峰度[K]）由两位经验丰富的放射科医生在后处理后计算。对于处理和对照小鼠的基线和每个时间点之间的所有参数的差异，使用Mann-Whitney检验。采用斯皮尔曼相关性检验评价DKI参数与相应病理性坏死分数（NF）之间的相关性。两组治疗后ADC值、D值、K值比较差异有统计学意义（P < 0.05）。治疗组ADC值、D值、K值在第7、14、21、28天与治疗前比较差异有统计学意义（P < 0.05）。DKI参数与NF之间存在显著相关性（ADC，r = 0.865，P < 0.001; D，r = 0.802，P < 0.001; K，r =-0.944，P < 0.001）。ILXPCI结果显示，治疗组的吸收面积大于对照组。DKI可用于评价TGF-β1抑制诱导的胃癌的全程治疗效果，ILXPCI技术将提高肿瘤显微结构分辨率。 证据等级：1技术有效性：第4阶段J. Magn. Reson。成像2019;49：1553-1564.
Accurate and complete response evaluation after treatment is important to implement individualized therapy for gastric cancer. To investigate the effectiveness of diffusion kurtosis imaging (DKI) and in‐line X‐ray phase contrast imaging (ILXPCI) in the assessment of the therapeutic efficacy by transforming growth factor beta 1 (TGF‐β1) inhibition. Prospective animal study. Thirty nude mice subcutaneous xenotransplantation tumor model of gastric cancer for DKI and 10 peritoneal metastasis nude mice model for ILXPCI. Examinations before and serially at 7, 14, 21, and 28 days after TGF‐β1 inhibition treatment were performed at 3T MRI including T2‐weighted imaging (T2WI) and DKI with five b values of 0, 500, 1000, 1500, 2000 s/mm2; ILXPCI examinations were performed at 14 days after treatment. DKI parameters (apparent diffusion coefficient [ADC], diffusivity [D] and kurtosis [K]) were calculated by two experienced radiologists after postprocessing. For the differences in all the parameters between the baseline and each timepoint for both the treated and the control mice, the Mann–Whitney test was used. The Spearman correlation test was used to evaluate correlations among the DKI parameters and corresponding pathologic necrosis fraction (NF). ADC, D, and K values were significantly different between the two groups after treatment (P < 0.05). Serial measurements in the treated group showed that the ADC, D, and K values were significantly different at 7, 14, 21, and 28 days compared with baseline (P < 0.05). There were significant correlations between DKI parameters and NF (ADC, r = 0.865, P < 0.001; D, r = 0.802, P < 0.001; K, r = –0.944, P < 0.001). The ILXPCI results in the treated group showed a stronger absorption area than the control group. DKI may be used to evaluate the complete course therapeutic effects of gastric cancer induced by TGF‐β1 inhibition, and the ILXPCI technique will improve the tumor microstructure resolution. Level of Evidence: 1 Technical Efficacy: Stage 4 J. Magn. Reson. Imaging 2019;49:1553–1564.
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影响因子：
8.4
作者：
Eisenhauer, E. A.;Therasse, P.;Verweij, J.
通讯作者：
Verweij, J.
影响因子：
8.4
作者：
Ferro, Ana;Peleteiro, Barbara;Lunet, Nuno
通讯作者：
Lunet, Nuno
影响因子：
19.7
作者：
Rosenkrantz, Andrew B.;Sigmund, Eric E.;Jensen, Jens H.
通讯作者：
Jensen, Jens H.
DOI：
10.1102/1470-7330.2012.9047
发表时间：
2012-09-28
期刊：
Cancer imaging : the official publication of the International Cancer Imaging Society
影响因子：
--
作者：
Bains LJ;Zweifel M;Thoeny HC
通讯作者：
Thoeny HC
DOI：
10.1102/1470-7330.2013.0023
发表时间：
2013-05-30
期刊：
Cancer imaging : the official publication of the International Cancer Imaging Society
影响因子：
--
作者：
Hallinan JT;Venkatesh SK
通讯作者：
Venkatesh SK