Complete sequences of two novel bla (NDM-1)-harbouring plasmids from two Acinetobacter towneri isolates in China associated with the acquisition of Tn125.
Complete sequences of two novel bla (NDM-1)-harbouring plasmids from two Acinetobacter towneri isolates in China associated with the acquisition of Tn125.
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来自中国两个汤氏不动杆菌分离株的两个新型 blaNDM-1 携带质粒的完整序列，与 Tn125 的获得相关
DOI：
10.1038/s41598-017-09624-0
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发表时间：
2017-08-24
影响因子：
4.6
通讯作者：
Huang L
中科院分区：
文献类型：
--
作者：
Zou D;Huang Y;Liu W;Yang Z;Dong D;Huang S;He X;Ao D;Liu N;Wang S;Wang Y;Tong Y;Yuan J;Huang L
两个新的新德里金属β-内酰胺酶-1(NDM-1)阳性质粒含有一个完整的复合转座子Tn125，分别来自两个不动杆菌分离株。从黄曲霉G165中分离到的pNDM-GJ01(30,293 )与已知的任何已知质粒结构没有同源性，除了含有bla NDM-1的转座子Tn125。在pNDM-GJ01中发现了一个新的epB基因和两个XRE型转录调控因子。从黄曲霉G295中分离到的pNDM-GJ02(62,011 )与pBJAB0715有最高的同源性(覆盖率为41%，核苷酸同源性为99%)。除了含有转座子Tn125的BLANDM-1外，pNDM-GJ02还含有一个IS26复合转座子，它含有ISCR1和两个携带不同盒阵列的1类整合子。两株临床分离株对β-内酰胺类抗生素高度耐药，对替吉环素和粘菌素敏感。在G295中检测到10个抗性基因，在G165中检测到1个抗性基因。所有供体均未通过肉汤配对和过滤配对获得接合体。在不动杆菌中出现了携带NDM-1的这两个新的质粒，pNDM-GJ01和pNDM-GJ02，这表明Tn125可移动整合。
Two novel New Delhi metallo-β-lactamase-1 (NDM-1)-positive plasmids containing a complete composite transposon, Tn125, from two respective Acinetobacter towneri isolates were characterized. Plasmid pNDM-GJ01 (30,293 bp) isolated from A. towneri G165 did not show homology to any known plasmid structure, except for the transposon Tn125 containing bla NDM-1. A novel repB gene and two XRE-type transcriptional regulators were found in pNDM-GJ01. Plasmid pNDM-GJ02 (62,011 bp) isolated from A. towneri G295 showed the highest homology to pBJAB0715 (41% coverage, 99% nucleotide identity). In addition to the bla NDM-1-harbouring transposon Tn125, pNDM-GJ02 also had an IS26-composite transposon, which contains ISCR1 and two class 1 integrons carrying different cassette arrays. Both clinical isolates were highly resistant to β-lactams and susceptible to tigecycline and colistin. Ten other resistance genes were detected in G295, and one other resistance gene was detected in G165. No transconjugant was obtained from any of the donors by broth and filter mating. The emergence of these two novel plasmids carrying NDM-1 in Acinetobacter spp., pNDM-GJ01 and pNDM-GJ02, suggests Tn125 mobile integration.
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影响因子：
4.9
作者：
Hujer, Kristine M.;Hujer, Andrea M.;Bonomo, Robert A.
通讯作者：
Bonomo, Robert A.
影响因子：
9.4
作者：
La Scola, B;Gundi, VAKB;Raoult, D
通讯作者：
Raoult, D
影响因子：
4.2
作者：
Liu, Y.;Wan, L. -G.;Xu, Q. -F.
通讯作者：
Xu, Q. -F.
影响因子：
5.2
作者：
Villa, Laura;Poirel, Laurent;Carattoli, Alessandra
通讯作者：
Carattoli, Alessandra
影响因子：
9.4
作者：
Chang, HC;Wei, YF;Chang, TC
通讯作者：
Chang, TC