Phenotypic and functional comparison of rat enteric neural crest-derived cells during fetal and early-postnatal stages.
Phenotypic and functional comparison of rat enteric neural crest-derived cells during fetal and early-postnatal stages.
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胎儿期和产后早期大鼠肠神经嵴来源细胞的表型和功能比较
DOI：
10.4103/1673-5374.310701
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发表时间：
2021-11
影响因子：
6.1
通讯作者：
Yu H
中科院分区：
文献类型：
--
作者：
Tian DH;Qin CH;Xu WY;Pan WK;Zhao YY;Zheng BJ;Chen XL;Liu Y;Gao Y;Yu H
在我们之前的研究中，我们发现随着培养时间的延长，肠神经嵴源性细胞（enteric neural crest-derived cells, ENCCs）的生长特性发生了变化，并且这些细胞在体外的增殖、迁移和神经分化潜力明显减弱。然而，在体外或体内还没有关于这些特征在胎儿和出生后早期发育差异的研究。在这项研究中，我们从大鼠的肠道中分离出胎儿（胚胎14.5天）和出生后（出生后2天）的ENCCs。与出生后的ENCCs相比，胎儿ENCCs具有更大的神经球最大横截面积、更强的迁移能力和更少的细胞凋亡。然而，胎儿和出生后的ENCCs具有相似的分化能力。胚胎和出生后的ENCCs在移植到巨结肠病大鼠模型后均存活。在这些患有先天性巨结肠病的大鼠中，与用出生后的ENCCs治疗的动物相比，用胎儿ENCCs治疗的动物的肌肠丛神经节细胞数量更高，远端肠压变化更大。这些发现表明，与出生后的ENCCs相比，胎儿ENCCs具有更高的存活、增殖和迁移能力，因此是治疗巨结肠病的更合适的种子细胞。本研究已于2016年3月3日获得西安交通大学第二附属医院动物伦理委员会批准（批准号2016086）。
In our previous study, we showed that with increasing time in culture, the growth characteristics of enteric neural crest-derived cells (ENCCs) change, and that the proliferation, migration and neural differentiation potential of these cells in vitro notably diminish. However, there are no studies on the developmental differences in these characteristics between fetal and early-postnatal stages in vitro or in vivo. In this study, we isolated fetal (embryonic day 14.5) and postnatal (postnatal day 2) ENCCs from the intestines of rats. Fetal ENCCs had greater maximum cross-sectional area of the neurospheres, stronger migration ability, and reduced apoptosis, compared with postnatal ENCCs. However, fetal and postnatal ENCCs had a similar differentiation ability. Fetal and postnatal ENCCs both survived after transplant into a rat model of Hirschsprung’s disease. In these rats with Hirschsprung’s disease, the number of ganglionic cells in the myenteric plexus was higher and the distal intestinal pressure change was greater in animals treated with fetal ENCCs compared with those treated with postnatal ENCCs. These findings suggest that, compared with postnatal ENCCs, fetal ENCCs exhibit higher survival and proliferation and migration abilities, and are therefore a more appropriate seed cell for the treatment of Hirschsprung’s disease. This study was approved by the Animal Ethics Committee of the Second Affiliated Hospital of Xi’an Jiaotong University (approval No. 2016086) on March 3, 2016.
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影响因子：
7.3
作者：
Nagy N;Goldstein AM
通讯作者：
Goldstein AM
影响因子：
3.6
作者：
Cheng, Lily S.;Hotta, Ryo;Goldstein, Allan M.
通讯作者：
Goldstein, Allan M.
DOI：
10.1152/ajpgi.00452.2012
发表时间：
2013-07-01
期刊：
American journal of physiology. Gastrointestinal and liver physiology
影响因子：
--
作者：
Lake JI;Heuckeroth RO
通讯作者：
Heuckeroth RO
在肠道中建立大脑：肠神经系统的发展。
影响因子：
3.5
作者：
Goldstein AM;Hofstra RM;Burns AJ
通讯作者：
Burns AJ
影响因子：
2.5
作者：
Sieber-Blum, M;Grim, M;Szeder, V
通讯作者：
Szeder, V