An oxygen-sensing mechanism for angiosperm adaptation to altitude.
An oxygen-sensing mechanism for angiosperm adaptation to altitude.
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DOI：
10.1038/s41586-022-04740-y
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发表时间：
2022-06
期刊：
影响因子：
64.8
通讯作者：
中科院分区：
文献类型：
--
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开花植物（被子植物）可以生长在极端的海拔高度，并被观察到生长在海拔6400米的地方；然而，使植物适应海拔的分子机制尚不清楚。海拔升高的一个显著特征是氧气分压（pO2）的降低。在这里，我们研究了海拔与叶绿素生物合成（需要分子氧）和缺氧相关基因表达相关的氧感之间的关系。我们发现，在被子植物的黄化幼苗中，光毒性叶绿素前体原叶绿素内酯的稳态水平受到大气氧浓度的影响。在拟南芥中，这是由植物半胱氨酸氧化酶（PCO） N-degron途径底物GROUP VII乙烯反应因子转录因子（ERFVIIs）介导的。erfvii正调控荧光IN BLUE LIGHT （FLU）的表达，后者抑制叶绿素生物合成的第一步，与受erfvii负调控的四吡啶合成酶形成失活复合物，从而抑制原叶绿素内酯。在代表不同被子植物支系的自然种群中，我们发现原生叶绿内酯的稳态水平、失活复合物组分的表达和缺氧相关基因的高度依赖于氧。最后，不同海拔拟南芥的ERFVII活性和积累表现出高度依赖性。因此，我们通过改变氧传感系统的敏感性，确定了一种遗传适应绝对海拔的机制。植物已经适应生长在特定的海拔高度，通过调节叶绿素合成来响应环境氧浓度，由海拔依赖的第七组乙烯响应因子的活性校准。
Flowering plants (angiosperms) can grow at extreme altitudes, and have been observed growing as high as 6,400 metres above sea level; however, the molecular mechanisms that enable plant adaptation specifically to altitude are unknown. One distinguishing feature of increasing altitude is a reduction in the partial pressure of oxygen (pO2). Here we investigated the relationship between altitude and oxygen sensing in relation to chlorophyll biosynthesis—which requires molecular oxygen—and hypoxia-related gene expression. We show that in etiolated seedlings of angiosperm species, steady-state levels of the phototoxic chlorophyll precursor protochlorophyllide are influenced by sensing of atmospheric oxygen concentration. In Arabidopsis thaliana, this is mediated by the PLANT CYSTEINE OXIDASE (PCO) N-degron pathway substrates GROUP VII ETHYLENE RESPONSE FACTOR transcription factors (ERFVIIs). ERFVIIs positively regulate expression of FLUORESCENT IN BLUE LIGHT (FLU), which represses the first committed step of chlorophyll biosynthesis, forming an inactivation complex with tetrapyrrole synthesis enzymes that are negatively regulated by ERFVIIs, thereby suppressing protochlorophyllide. In natural populations representing diverse angiosperm clades, we find oxygen-dependent altitudinal clines for steady-state levels of protochlorophyllide, expression of inactivation complex components and hypoxia-related genes. Finally, A. thaliana accessions from contrasting altitudes display altitude-dependent ERFVII activity and accumulation. We thus identify a mechanism for genetic adaptation to absolute altitude through alteration of the sensitivity of the oxygen-sensing system. Plants have adapted to grow at specific altitudes by regulating chlorophyll synthesis in response to ambient oxygen concentration, calibrated by altitude-dependent activity of GROUP VII ETHYLENE RESPONSE FACTOR.
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影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
64.8
作者：
Jarvis, David E.;Ho, Yung Shwen;Tester, Mark
通讯作者：
Tester, Mark
影响因子：
7.2
作者：
Hou, Zhiwei;Yang, Yanyu;Grimm, Bernhard
通讯作者：
Grimm, Bernhard
影响因子：
16
作者：
Gibbs, Daniel J.;Isa, Nurulhikma Md;Movahedi, Mahsa;Lozano-Juste, Jorge;Mendiondo, Guillermina M.;Berckhan, Sophie;Marin-de la Rosa, Nora;Conde, Jorge Vicente;Correia, Cristina Sousa;Pearce, Simon P.;Bassel, George W.;Hamali, Bulut;Talloji, Prabhavathi;Tome, Daniel F. A.;Coego, Alberto;Beynon, Jim;Alabadi, David;Bachmair, Andreas;Leon, Jose;Gray, Julie E.;Theodoulou, Frederica L.;Holdsworth, Michael J.
通讯作者：
Holdsworth, Michael J.
影响因子：
3.6
作者：
Angel R;Conrad R;Dvorsky M;Kopecky M;Kotilínek M;Hiiesalu I;Schweingruber F;Doležal J
通讯作者：
Doležal J