Culture-free diagnostics of Pseudomonas aeruginosa infection by silver nanorod array based SERS from clinical sputum samples.
Culture-free diagnostics of Pseudomonas aeruginosa infection by silver nanorod array based SERS from clinical sputum samples.
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DOI：
10.1016/j.nano.2014.04.010
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发表时间：
2014-11
影响因子：
5.4
通讯作者：
Zughaier, Susu M.
中科院分区：
文献类型：
--
作者：
Wu, Xiaomeng;Chen, Jing;Li, Xibo;Zhao, Yiping;Zughaier, Susu M.
关键词：
铜绿假单胞菌可引起免疫功能低下患者的严重感染，感染的成功抗生素治疗依赖于准确和快速识别感染因子。本文报道了一种基于绿脓杆菌主要生物标志物绿脓菌素（PCN）的表面增强拉曼光谱（Sers）的免培养诊断方法。该平台可以检测水溶液和加标临床痰液样本中低至5 ppb或2.38 × 10−8 mol L−1的PCN。它还被用于动态监测铜绿假单胞菌在其生长过程中的PCN排泄。15例临床痰标本中检测到PCN，提示铜绿假单胞菌感染，灵敏度为95.6%，特异性为93.3%。该系统可以有利地快速处理多个样本，克服了对细菌培养和诊断微生物学测定的需要，并且在铜绿假单胞菌感染的早期检测中具有广泛的意义。
Pseudomonas aeruginosa can cause major infection in immunocompromised patients, and successful antibiotic treatment of the infection relies on accurate and rapid identification of the infectious agents. Here, we reported a culture-free diagnostic method based on the surface-enhanced Raman spectroscopy (SERS) of pyocyanin (PCN), a major biomarker of P. aeruginosa. This platform can detect PCN as low as 5 ppb or 2.38 × 10−8 mol L−1 in both aqueous solutions and spiked clinical sputum samples. It has also been used to dynamically monitor the excretion of PCN by P. aeruginosa during its growth. The presence of PCN has been detected by SERS in 15 clinical sputum samples, which indicates P. aeruginosa infection, with 95.6% sensitivity and 93.3% specificity. The system can advantageously process multiple specimens rapidly, overcomes the need for bacterial culture and diagnostic microbiology assays, and have widespread implications in the early detection of P. aeruginosa infection.
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影响因子：
4
作者：
Chaney, SB;Shanmukh, S;Zhao, YP
通讯作者：
Zhao, YP
使用金纳米颗粒固定基质对细菌生物标志物进行表面增强拉曼光谱检测
影响因子：
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作者：
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影响因子：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
3.6
作者：
Dietrich, Lars E. P.;Price-Whelan, Alexa;Newman, Dianne K.
通讯作者：
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