Characterization of Staphylococcus aureus Isolated from Non-Native Patients with Skin and Soft Tissue Infections in Shanghai.
Characterization of Staphylococcus aureus Isolated from Non-Native Patients with Skin and Soft Tissue Infections in Shanghai.
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上海外地皮肤软组织感染患者金黄色葡萄球菌特征分析
DOI：
10.1371/journal.pone.0123557
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Han LZ
中科院分区：
文献类型：
--
作者：
Gu FF;Hou Q;Yang HH;Zhu YQ;Guo XK;Ni YX;Han LZ
背景金黄色葡萄球菌是引起皮肤和软组织感染的主要原因之一，但目前关于金黄色葡萄球菌的特征性研究还很少。金黄色葡萄球菌从非本地患者与SSTI在中国。方法对52例非本地人S。金黄色葡萄球菌SSTI和65例金黄色葡萄球菌SSTI本地对照患者。金黄色葡萄球菌SSTI。52和65 S。分别从两组中收集金黄色葡萄球菌分离株。S.对分离的金黄色葡萄球菌进行了药敏试验、毒素基因检测和序列型、spa型、agr型和SCCmec型的分子分型。结果耐甲氧西林S.金黄色葡萄球菌（MRSA）的检测在8个非本地患者和14个本地SSTI患者。总体而言，S.发现从非本地患者中分离的金黄色葡萄球菌高于本地患者。共在14株分离株（4株来自非本地患者; 10株来自本地患者）中发现CC 59（ST 338和ST 59），其中9株携带lukS/F-PV（3株来自非本地患者; 6株来自本地患者）。在12个分离株中发现了ST 7，所有12个分离株均在本地患者中发现。在11个分离株（6个来自非本地患者; 5个来自本地患者）和5个ST 398 lukS/F-PV阳性甲氧西林敏感S.金黄色葡萄球菌（MSSA）均在非本地患者中发现。对S.分离自非本地患者的金黄色葡萄球菌与分离自本地患者的金黄色葡萄球菌有很大不同。与本地患者相比，lukS/F-PV在源自患有SSTI的非本地患者的分离株中更常见（31对7，P <0.0001）。结论CC 59是上海地区SSTI患者中最常见的克隆复合体。其他最常见的序列类型是ST 7和牲畜ST 398。对S.分离自非本地患者的金黄色葡萄球菌与分离自本地患者的金黄色葡萄球菌有很大不同。S.分离自非本地患者的金黄色葡萄球菌更可能携带lukS/F-PV。
Background Staphylococcus aureus is one predominant cause of skin and soft-tissue infections (SSTIs), but little information exists regarding the characterization of S. aureus from non-native patients with SSTIs in China. Methods In this study, we enrolled 52 non-native patients with S. aureus SSTIs, and 65 native control patients with S. aureus SSTIs in Shanghai. 52 and 65 S. aureus isolates were collected from both groups, respectively. S. aureus isolates were characterized by antimicrobial susceptibility testing, toxin gene detection, and molecular typing with sequence type, spa type, agr group and SCCmec type. Results Methicillin-resistant S. aureus (MRSA) was detected in 8 non-native patients and 14 native patients with SSTIs. Overall, antimicrobial susceptibilities of S. aureus isolated from non-native patients were found higher than those from native patients. CC59 (ST338 and ST59) was found in a total of 14 isolates (4 from non-native patients; 10 from native patients), 9 of which were carrying lukS/F-PV (3 from non-native patients; 6 from native patients). ST7 was found in 12 isolates and all 12 isolates were found in native patients. The livestock-associated clone ST398 was found in 11 isolates (6 from non-native patients; 5 from native patients), and 5 ST398 lukS/F-PV-positive methicillin-susceptible S. aureus (MSSA) were all discovered among non-native patients. The molecular epidemiology of S. aureus isolated from non-native patients was quite different from those from native patients. lukS/F-PV was more frequent in isolates originating from non-native patients with SSTIs compared to native patients (31 vs. 7, P <0.0001). Conclusions CC59 was the most common clonal complex among patients with SSTIs in Shanghai. The other most common sequence types were ST7 and Livestock ST398. The molecular epidemiology of S. aureus isolated from non-native patients was quite different from those from native patients. S. aureus isolated from non-native patients was more likely to carry lukS/F-PV.
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DOI：
10.1016/j.ijmm.2013.02.007
发表时间：
2013-08
期刊：
International journal of medical microbiology : IJMM
影响因子：
--
作者：
Otto M
通讯作者：
Otto M
影响因子：
2.4
作者：
Borgundvaag, Bjug;Wil Ng;Katz, Kevin
通讯作者：
Katz, Kevin
影响因子：
9.4
作者：
Kaltsas, Anna;Guh, Alice;Fries, Bettina C.
通讯作者：
Fries, Bettina C.
影响因子：
3.7
作者：
Qiao Y;Ning X;Chen Q;Zhao R;Song W;Zheng Y;Dong F;Li S;Li J;Wang L;Zeng T;Dong Y;Yao K;Yu S;Yang Y;Shen X
通讯作者：
Shen X
影响因子：
11.8
作者：
Tattevin, Pierre;Schwartz, Brian S.;Binh An Diep
通讯作者：
Binh An Diep