Multiple episodic evolution events in V1R receptor genes of East-African cichlids.
Multiple episodic evolution events in V1R receptor genes of East-African cichlids.
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DOI：
10.1093/gbe/evu086
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发表时间：
2014-05-06
影响因子：
3.3
通讯作者：
Okada N
中科院分区：
文献类型：
--
作者：
Nikaido M;Ota T;Hirata T;Suzuki H;Satta Y;Aibara M;Mzighani SI;Sturmbauer C;Hagino-Yamagishi K;Okada N
鱼类用嗅觉来探测各种不易挥发的化学信号，因此，这种感觉是生存和交流的关键。然而，嗅觉在慈鲷的社会互动，尤其是生殖互动中的作用仍然存在争议。为了深入了解这一问题，我们研究了东非稚鱼中编码v1rs的基因，v1rs是生殖信息素受体的可能候选基因。有趣的是，我们发现在多个慈鲷谱系中，6个V1R基因中有4个的非同义替换多于同义替换。首先，我们发现，在坦噶尼喀湖物种群辐射之前，通过非同义替换的偶发性积累，生活在东非大湖的所有鲷类中都存在高度二态的V1R2等位基因群。我们进一步在trophheini部落的V1R1， Trematocarini部落的V1R3和V1R6中检测到这种偶发事件。在这些例子中，非同义替换的过剩量表示为dN/dS > 1，经Fisher精确检验均具有统计学显著性。此外，我们推测这些偶发性事件中的氨基酸变化可能是功能开关，因为它们发生在假定的配体结合口袋中。我们在一个独特的基因家族中发现了多个偶发事件的发生和意想不到的基因多样性，这提示了V1R对慈鲷物种多样化和社会互动的贡献。
Fish use olfaction to detect a variety of nonvolatile chemical signals, and thus, this sense is key to survival and communication. However, the contribution of the olfactory sense to social—especially reproductive—interactions in cichlids is still controversial. To obtain insights into this issue, we investigated the genes encoding V1Rs—possible candidates for reproductive pheromone receptors—among East-African cichlids. Interestingly, we found an excess of nonsynonymous over synonymous substitutions in four of six V1R genes in multiple cichlid lineages. First, we found that highly dimorphic V1R2 allele groups were shared among the cichlids inhabiting all East-African Great Lakes emerged through the episodic accumulation of the nonsynonymous substitutions prior to the radiation of the Lake Tanganyika species flock. We further detected such episodic events in V1R1 of the tribe Tropheini, and in V1R3 and V1R6 of the tribe Trematocarini. The excess of nonsynonymous substitutions in these examples were indicated as dN/dS > 1, which were all statistically significant by Fisher’s exact test. Furthermore, we speculate that the amino acid changes in these episodic events are likely functional switch because they occurred in the putative ligand-binding pocket. Our finding of the occurrence of multiple episodic events and the unexpected gene diversity in one unique gene family is suggestive of the contribution of the V1R to the species diversification and the social interaction in cichlids.
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影响因子：
7
作者：
Nikaido M;Noguchi H;Nishihara H;Toyoda A;Suzuki Y;Kajitani R;Suzuki H;Okuno M;Aibara M;Ngatunga BP;Mzighani SI;Kalombo HW;Masengi KW;Tuda J;Nogami S;Maeda R;Iwata M;Abe Y;Fujimura K;Okabe M;Amano T;Maeno A;Shiroishi T;Itoh T;Sugano S;Kohara Y;Fujiyama A;Okada N
通讯作者：
Okada N
影响因子：
3.3
作者：
Nikaido M;Suzuki H;Toyoda A;Fujiyama A;Hagino-Yamagishi K;Kocher TD;Carleton K;Okada N
通讯作者：
Okada N
影响因子：
5.8
作者：
Posada, D;Crandall, KA
通讯作者：
Crandall, KA
影响因子：
4.9
作者：
Clement, M;Posada, D;Crandall, KA
通讯作者：
Crandall, KA
影响因子：
10.7
作者：
Samonte, Irene E.;Satta, Yoko;Klein, Jan
通讯作者：
Klein, Jan