Lineage-specific expansion of vomeronasal type 2 receptor-like (OlfC) genes in cichlids may contribute to diversification of amino acid detection systems.
Lineage-specific expansion of vomeronasal type 2 receptor-like (OlfC) genes in cichlids may contribute to diversification of amino acid detection systems.
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DOI：
10.1093/gbe/evt041
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发表时间：
2013
影响因子：
3.3
通讯作者：
Okada N
中科院分区：
文献类型：
--
作者：
Nikaido M;Suzuki H;Toyoda A;Fujiyama A;Hagino-Yamagishi K;Kocher TD;Carleton K;Okada N
鱼利用嗅觉来感知水中各种不挥发的化学信号。然而，物种丰富的慈鱼的嗅觉在进化上的重要性是有争议的。在这里，我们利用维多利亚湖叶鱼的细菌人工染色体文库，测定了犁鼻2型受体样(OlfC：硬骨鱼中推定的氨基酸受体)基因簇的几乎完整的序列。在该簇区，我们发现了61个完整的OlfC基因，这是迄今为止在所研究的7种硬骨鱼中发现的最多的OlfC基因。尼罗罗非鱼基因组草稿序列的数据挖掘和基因组Southern杂交分析表明，所有现代罗非鱼的祖先都已经开发出几乎相同的olfC基因谱系，这是通过谱系特异的基因扩展完成的。此外，受体序列的比较表明，最近复制的并列基因在预测参与氨基酸选择性的特定位点上比不同物种的直系同源基因更具变异性。因此，通过基因扩增增加平行对数可能会导致氨基酸检测的功能多样化。这项研究表明，慈鱼已经形成了通过OlfCs检测各种氨基酸(及其衍生物)的强大能力，这可能是它们摄食生境异常多样化的原因之一。
Fish use olfaction to sense a variety of nonvolatile chemical signals in water. However, the evolutionary importance of olfaction in species-rich cichlids is controversial. Here, we determined an almost complete sequence of the vomeronasal type 2 receptor-like (OlfC: putative amino acids receptor in teleosts) gene cluster using the bacterial artificial chromosome library of the Lake Victoria cichlid, Haplochromis chilotes. In the cluster region, we found 61 intact OlfC genes, which is the largest number of OlfC genes identified among the seven teleost fish investigated to date. Data mining of the Oreochromis niloticus (Nile tilapia) draft genome sequence, and genomic Southern hybridization analysis revealed that the ancestor of all modern cichlids had already developed almost the same OlfC gene repertoire, which was accomplished by lineage-specific gene expansions. Furthermore, comparison of receptor sequences showed that recently duplicated paralogs are more variable than orthologs of different species at particular sites that were predicted to be involved in amino acid selectivity. Thus, the increase of paralogs through gene expansion may lead to functional diversification in detection of amino acids. This study implies that cichlids have developed a potent capacity to detect a variety of amino acids (and their derivatives) through OlfCs, which may have contributed to the extraordinary diversity of their feeding habitats.
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影响因子：
16.2
作者：
Friedrich, RW;Korsching, SI
通讯作者：
Korsching, SI
DOI：
10.1073/pnas.0900470106
发表时间：
2009-06-16
影响因子：
11.1
作者：
Koide, Tetsuya;Miyasaka, Nobuhiko;Yoshihara, Yoshihiro
通讯作者：
Yoshihara, Yoshihiro
影响因子：
3.7
作者：
Hashiguchi, Yasuyuki;Furuta, Yoshimi;Nishida, Mutsumi
通讯作者：
Nishida, Mutsumi
DOI：
10.1073/pnas.95.20.11987
发表时间：
1998-09-29
影响因子：
11.1
作者：
Cao, YX;Oh, BC;Stryer, L
通讯作者：
Stryer, L
影响因子：
64.8
作者：
Kimoto, H;Haga, S;Touhara, K
通讯作者：
Touhara, K