Genome-wide expression profiling of maize in response to individual and combined water and nitrogen stresses.
Genome-wide expression profiling of maize in response to individual and combined water and nitrogen stresses.
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DOI：
10.1186/1471-2164-14-3
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发表时间：
2013-01-16
期刊：
影响因子：
4.4
通讯作者：
Rothstein SJ
中科院分区：
文献类型：
--
作者：
Humbert S;Subedi S;Cohn J;Zeng B;Bi YM;Chen X;Zhu T;McNicholas PD;Rothstein SJ
水和氮是实现现代玉米品种高产潜力所需的两种最关键的投入。然而，在大多数农业环境下，它们往往稀缺且昂贵。幸运的是，在过去的几十年里，在帮助种植者决策过程的建模方面取得了巨大的进步，现在有许多工具可以在环境和经济上实现更可持续的做法。然而，我们在野外观察到的氮和水胁迫下的生理变化的经验知识与我们对导致这些变化的分子过程的有限理解之间仍然存在很大差距。这项工作特别研究了同时应激对转录组的影响。在温室环境中，玉米植株在严格控制的氮和水条件下生长，允许对各种组织和胁迫组合进行采样。使用该材料进行的微阵列分析实验表明，氮和水限制的同时存在对基因表达的影响程度远远大于预期。聚类分析还揭示了两种胁迫之间的相互作用如何在不同水平的水分胁迫和恢复中形成基因表达模式。总的来说，这项研究表明，转录组上特定应激组合的分子特征可能与个体应激的影响一样独特，因此强调了从个体应激反应研究中推断出更复杂环境的结论的难度。
Water and nitrogen are two of the most critical inputs required to achieve the high yield potential of modern corn varieties. Under most agricultural settings however they are often scarce and costly. Fortunately, tremendous progress has been made in the past decades in terms of modeling to assist growers in the decision making process and many tools are now available to achieve more sustainable practices both environmentally and economically. Nevertheless large gaps remain between our empirical knowledge of the physiological changes observed in the field in response to nitrogen and water stresses, and our limited understanding of the molecular processes leading to those changes. This work examines in particular the impact of simultaneous stresses on the transcriptome. In a greenhouse setting, corn plants were grown under tightly controlled nitrogen and water conditions, allowing sampling of various tissues and stress combinations. A microarray profiling experiment was performed using this material and showed that the concomitant presence of nitrogen and water limitation affects gene expression to an extent much larger than anticipated. A clustering analysis also revealed how the interaction between the two stresses shapes the patterns of gene expression over various levels of water stresses and recovery. Overall, this study suggests that the molecular signature of a specific combination of stresses on the transcriptome might be as unique as the impact of individual stresses, and hence underlines the difficulty to extrapolate conclusions obtained from the study of individual stress responses to more complex settings.
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