Isolation of entomopathogenic fungi from soils and Ixodes scapularis (Acari: Ixodidae) ticks: prevalence and methods.
Isolation of entomopathogenic fungi from soils and Ixodes scapularis (Acari: Ixodidae) ticks: prevalence and methods.
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DOI：
10.1603/033.046.0321
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发表时间：
2009-05
影响因子：
2.1
通讯作者：
Stafford KC 3rd
中科院分区：
文献类型：
--
作者：
Tuininga AR;Miller JL;Morath SU;Daniels TJ;Falco RC;Marchese M;Sahabi S;Rosa D;Stafford KC 3rd
昆虫病原真菌通常存在于提供蜱虫栖息地的森林土壤中，许多物种对黑腿蜱（Ixodes scapularis Say）具有致病性。作为发展有效的生物防治策略的第一步，本研究的目的是确定从东部落叶林的土壤和蜱类样品中分离昆虫病原真菌的最佳方法。肩胛肌是常见的。评估了几种方法：（1）将土壤、落叶和蜱接种在两种类型的培养基上，（2）使用Galleria诱饵法测定土壤中的昆虫病原真菌，（3）从从土壤、Galleria和蜱获得的纯培养物中提取核核糖体重复序列的内转录间隔区（ITS）的DNA，并进行扩增和测序，（4）从土壤、Galleria和蜱获得的纯培养物中提取DNA，并进行测序。（4）直接从蜱中提取DNA，扩增并测序。我们得出结论：（1）蜱在土壤中遇到潜在昆虫病原真菌的频率高于落叶，（2）土壤中发现的许多潜在昆虫病原真菌都可以很容易地培养，（3）Galleria诱饵法是从土壤中分离这些真菌的有效方法，（4）尽管由于样本量小，本研究中无法从蜱中提取DNA，从土壤和蜱中分离的真菌DNA提取是成功的，并分别在100%和73%的样品中提供干净的序列。上述方法的组合显然是必要的最佳表征昆虫病原真菌与蜱在环境中。
Entomopathogenic fungi are commonly found in forested soils that provide tick habitat, and many species are pathogenic to Ixodes scapularis Say, the blacklegged tick. As a first step to developing effective biocontrol strategies, the objective of this study was to determine the best methods to isolate entomopathogenic fungal species from field-collected samples of soils and ticks from an Eastern deciduous forest where I. scapularis is common. Several methods were assessed: (1) soils, leaf litter, and ticks were plated on two types of media; (2) soils were assayed for entomopathogenic fungi using the Galleria bait method; (3) DNA from internal transcribed spacer (ITS) regions of the nuclear ribosomal repeat was extracted from pure cultures obtained from soils, Galleria, and ticks and was amplified and sequenced; and (4) DNA was extracted directly from ticks, amplified, and sequenced. We conclude that (1) ticks encounter potentially entomopathogenic fungi more often in soil than in leaf litter, (2) many species of potentially entomopathogenic fungi found in the soil can readily be cultured, (3) the Galleria bait method is a sufficiently efficient method for isolation of these fungi from soils, and (4) although DNA extraction from ticks was not possible in this study because of small sample size, DNA extraction from fungi isolated from soils and from ticks was successful and provided clean sequences in 100 and 73% of samples, respectively. A combination of the above methods is clearly necessary for optimal characterization of entomopathogenic fungi associated with ticks in the environment.
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影响因子：
1.3
作者：
Basso, W;Venturini, L;Dubey, JP
通讯作者：
Dubey, JP
影响因子：
2.1
作者：
Kirkland, BH;Westwood, GS;Keyhani, NO
通讯作者：
Keyhani, NO
影响因子：
2.1
作者：
Dohm, DJ;Rowton, ED;Turell, MJ
通讯作者：
Turell, MJ
影响因子：
4.9
作者：
Bridge, P;Spooner, B
通讯作者：
Spooner, B
影响因子：
2.1
作者：
Daniels, TJ;Falco, RC;Fish, D
通讯作者：
Fish, D