Contrasting expression of keratins in mouse and human embryonic stem cells.
Contrasting expression of keratins in mouse and human embryonic stem cells.
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DOI：
10.1371/journal.pone.0003451
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发表时间：
2008
期刊：
影响因子：
3.7
通讯作者：
Oshima RG
中科院分区：
文献类型：
--
作者：
Maurer J;Nelson B;Ceceña G;Bajpai R;Mercola M;Terskikh A;Oshima RG
RNA表达数据表明，人胚胎干细胞（hES）细胞角蛋白中间丝蛋白的RNA表达不同于小鼠ES细胞（mES）。这些差异在细胞和蛋白质水平得到证实，可能反映了小鼠和人胚泡来源的胚胎干细胞上皮性质的根本差异。小鼠ES细胞表达非常低水平的简单上皮角蛋白K8、K18和K19。相比之下，hES细胞表达中等水平的这些中间丝蛋白的RNA，如源自小鼠上胚层的小鼠干细胞一样。在小鼠和人ES细胞培养物中，K8和K18 RNA的表达与c-Jun RNA表达增加相关。然而，与分化为神经元祖细胞相关的K8和K18表达降低与Snai 2（Slug）转录抑制的表达增加相关，而不是Jun表达降低。增加K7表达与增加CDX 2和减少Oct 4 RNA表达相关，Oct 4 RNA表达与hES细胞形成滋养层衍生物相关。我们的研究支持这样的观点，即hES细胞比小鼠ES细胞更类似于小鼠上胚层细胞，并且与hES细胞的上皮性质一致。角蛋白中间丝在hES细胞中的表达可能调节对死亡受体介导的凋亡和应激的敏感性。
RNA expression data reveals that human embryonic stem (hES) cells differ from mouse ES (mES) cells in the expression of RNAs for keratin intermediate filament proteins. These differences were confirmed at the cellular and protein level and may reflect a fundamental difference in the epithelial nature of embryonic stem cells derived from mouse and human blastocysts. Mouse ES cells express very low levels of the simple epithelial keratins K8, K18 and K19. By contrast hES cells express moderate levels of the RNAs for these intermediate filament proteins as do mouse stem cells derived from the mouse epiblast. Expression of K8 and K18 RNAs are correlated with increased c-Jun RNA expression in both mouse and human ES cell cultures. However, decreasing K8 and K18 expression associated with differentiation to neuronal progenitor cells is correlated with increasing expression of the Snai2 (Slug) transcriptional repression and not decreased Jun expression. Increasing K7 expression is correlated with increased CDX2 and decreased Oct4 RNA expression associated with the formation of trophoblast derivatives by hES cells. Our study supports the view that hES cells are more similar to mouse epiblast cells than mouse ES cells and is consistent with the epithelial nature of hES cells. Keratin intermediate filament expression in hES cells may modulate sensitivity to death receptor mediated apoptosis and stress.
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DOI：
10.1073/pnas.77.7.4113
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
BRULET, P;BABINET, C;JACOB, F
通讯作者：
JACOB, F
影响因子：
64.8
作者：
Brons, I. Gabrielle M.;Smithers, Lucy E.;Vallier, Ludovic
通讯作者：
Vallier, Ludovic
影响因子：
64.5
作者：
Niwa, H;Toyooka, T;Rossant, J
通讯作者：
Rossant, J
影响因子：
64.5
作者：
Kim, Tae Hoon;Abdullaev, Ziedulla K.;Ren, Bing
通讯作者：
Ren, Bing
DOI：
10.1083/jcb.200602146
发表时间：
2006-07-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ku NO;Omary MB
通讯作者：
Omary MB