Characterization of a Zika Virus Isolate from Colombia.
Characterization of a Zika Virus Isolate from Colombia.
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DOI：
10.1371/journal.pntd.0005019
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发表时间：
2016-09
影响因子：
3.8
通讯作者：
Rico-Hesse R
中科院分区：
文献类型：
--
作者：
Lahon A;Arya RP;Kneubehl AR;Vogt MB;Dailey Garnes NJ;Rico-Hesse R
寨卡病毒（黄病毒属）是已知的第一种蚊媒病毒，可导致人类小头畸形和流产率高。通常情况下，寨卡病毒会引起自限性全身性疾病;然而，目前寨卡病毒在美洲的爆发与胎儿畸形和格林-巴利综合征的发病率增加有关。文献中描述的寨卡病毒分离株非常少，需要活病毒来进行发病机制研究并开发疫苗和治疗方法。我们直接从哥伦比亚巴兰基利亚感染者的血清中分离出寨卡病毒株FLR（2015年12月）。在这里，我们描述了病人的临床过程和特点菌株FLR在蚊子和哺乳动物细胞中的生长特性和对紫外线灭活的部分抗性。还分析了FLR的全基因组序列（包括3'非翻译区），以确定其可能的地理来源，并查明与其他寨卡病毒株的结构差异。我们预计，对这种可供全球分布的寨卡病毒低传代临床分离株的研究，将有助于揭示病毒复制和宿主免疫反应的机制，这些机制导致了目前美洲流行期间出现的各种严重临床表现。寨卡病毒（ZIKV）是一种蚊子传播的病毒，会引起发热性疾病，其特征是明显的皮疹和偶尔的关节痛。ZIKV感染的严重并发症很少报告，直到2015年，巴西官员首次报告了ZIKV感染与小头畸形和格林-巴利综合征急剧增加之间的关联。从那时起，ZIKV已经在美洲的39个国家被发现。因此，来自当前爆发的ZIKV毒株的鉴定和表征是至关重要的。在这项研究中，我们成功地从一个人身上分离出了ZIKV毒株（ZIKV-FLR），这个人在哥伦比亚一个有登革热、基孔肯雅热和ZIKV传播的地区旅行时被蚊子叮咬。我们对全基因组序列的分析显示，这种病毒株可能起源于加勒比地区，而不是直接从巴西进口的。我们还发现，这种病毒株是部分抵抗灭活紫外线照射，相比登革热病毒。需要进一步的工作来阐明这种增加的抵抗力和导致更严重疾病的潜力背后的潜在机制。
Zika virus (Flavivirus genus) is the first mosquito-borne virus known to cause high rates of microcephaly and abortion in humans. Typically, Zika virus causes a self-limiting, systemic illness; however, the current outbreak of Zika virus in the Americas has been associated with increased rates of fetal malformations and Guillain-Barré syndrome. Very few Zika virus isolates have been described in the literature, and live viruses are needed to perform studies of pathogenesis and to develop vaccines and treatments. We isolated Zika virus, strain FLR, directly from the serum of an individual infected in Barranquilla, Colombia (December, 2015). Here, we describe the patient’s clinical course and characterize strain FLR by its growth characteristics in mosquito and mammalian cells and its partial resistance to UV-inactivation. The full genome sequence of FLR was also analyzed (including the 3’ un-translated region), to determine its probable geographic origin, and to pinpoint structural differences from other Zika virus strains. We anticipate that the study of this low passage, clinical isolate of Zika virus, which is available for worldwide distribution, will help uncover the mechanisms of viral replication and host immune responses contributing to the varied and sometimes severe clinical presentations seen during the current epidemic in the Americas. Zika virus (ZIKV) is a mosquito-transmitted virus that causes a febrile illness characterized by a pronounced rash and occasional arthralgia. Serious complications from ZIKV infections were rarely reported until 2015, when Brazilian officials first reported an association between ZIKV infections and a dramatic increase in microcephaly and Guillian-Barré syndrome. Since then, ZIKV has been found in 39 countries in the Americas. Thus, the identification and characterization of ZIKV strains from the current outbreak are of utmost importance. In this study, we successfully isolated a ZIKV strain (ZIKV-FLR) from an individual who was bitten by mosquitoes while traveling in an area of Colombia that had ongoing dengue, chikungunya, and ZIKV transmission. Our analysis of the full genome sequence revealed that this virus strain may have originated in the Caribbean and was not directly imported from Brazil. We also discovered that this virus strain was partially resistant to inactivation by ultraviolet irradiation, when compared to dengue virus. Further work is needed to elucidate the underlying mechanisms behind this increased resistance and potential to cause more severe disease.
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DOI：
10.3390/v3091739
发表时间：
2011-09
期刊：
Viruses
影响因子：
--
作者：
Gebhard LG;Filomatori CV;Gamarnik AV
通讯作者：
Gamarnik AV
DOI：
10.1056/nejmoa1602412
发表时间：
2016-12-15
期刊：
The New England journal of medicine
影响因子：
--
作者：
Brasil P;Pereira JP Jr;Moreira ME;Ribeiro Nogueira RM;Damasceno L;Wakimoto M;Rabello RS;Valderramos SG;Halai UA;Salles TS;Zin AA;Horovitz D;Daltro P;Boechat M;Raja Gabaglia C;Carvalho de Sequeira P;Pilotto JH;Medialdea-Carrera R;Cotrim da Cunha D;Abreu de Carvalho LM;Pone M;Machado Siqueira A;Calvet GA;Rodrigues Baião AE;Neves ES;Nassar de Carvalho PR;Hasue RH;Marschik PB;Einspieler C;Janzen C;Cherry JD;Bispo de Filippis AM;Nielsen-Saines K
通讯作者：
Nielsen-Saines K
影响因子：
5.4
作者：
Mota, Javier;Rico-Hesse, Rebeca
通讯作者：
Rico-Hesse, Rebeca
影响因子：
5.4
作者：
Cologna, R;Armstrong, PM;Rico-Hesse, R
通讯作者：
Rico-Hesse, R
影响因子：
64.8
作者：
Kostyuchenko, Victor A.;Lim, Elisa X. Y.;Lok, Shee-Mei
通讯作者：
Lok, Shee-Mei