Correlated Positioning of Homologous Chromosomes in Daughter Fibroblast Cells
Correlated Positioning of Homologous Chromosomes in Daughter Fibroblast Cells
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子代成纤维细胞同源染色体的相关定位
DOI：
10.1023/a:1009279918034
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发表时间：
2004
影响因子：
2.6
通讯作者：
H. Yokota
中科院分区：
文献类型：
--
作者：
H. Sun;H. Yokota
一种新的方法分析染色体分离对女儿的人成纤维细胞显示，染色体在子细胞核中的定位是密切相关的，它们的相对位置在G1间期。两个地形值，即距离和一对同源染色体之间的角间距，确定使用荧光原位杂交与四个不同的着丝粒DNA探针。这些地形值作为一个群体表现出广泛的分布，但是，令我们惊讶的是，两者在来自同一母细胞（子细胞）的每对细胞内都有很强的相关性。这种相关性不受子细胞之间的细胞间距离的影响。我们在这份报告中表明，染色体在G1间期的定位主要是由它们的配置在有丝分裂，与核结构模型，其中染色体是固定的，在全球范围内的G1间期细胞核一致。
A new method of analyzing chromosome segregation in pairs of daughter human fibroblasts revealed that the positioning of chromosomes in daughter nuclei was closely correlated with their relative positions during the G1 interphase. Two topographic values, namely distance and the angular separation between a pair of homologous chromosomes, were determined using fluorescence in-situ hybridization with four different centromeric DNA probes. These topographical values exhibited a broad distribution as a population, but, to our surprise, both were strongly correlated within each pair of cells derived from the same mother cell (daughters). This correlation was not affected by cell-to-cell distances between daughter cells. We demonstrate in this report that the positioning of chromosomes at G1 interphase is chiefly determined by their configuration at mitosis, consistent with the nuclear architecture model in which chromosomes are immobile at a global scale in the G1 interphase nuclei.
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使用延时共焦显微镜分析人类细胞的着丝粒动态。
DOI：
--
发表时间：
1999
期刊：
Methods in cell biology.
影响因子：
--
作者：
Sullivan,KF;Shelby,RD
通讯作者：
Shelby,RD
DOI：
10.1073/pnas.94.3.849
发表时间：
1997-02-04
影响因子：
11.1
作者：
Maniotis, AJ;Chen, CS;Ingber, DE
通讯作者：
Ingber, DE
影响因子：
3.7
作者：
Spector,DL
通讯作者：
Spector,DL
DOI：
10.1073/pnas.85.23.9138
发表时间：
1988-12-01
影响因子：
11.1
作者：
PINKEL, D;LANDEGENT, J;GRAY, J
通讯作者：
GRAY, J