Characterization of the receptors for mycobacterial cord factor in Guinea pig.
Characterization of the receptors for mycobacterial cord factor in Guinea pig.
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DOI：
10.1371/journal.pone.0088747
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Yamasaki S
中科院分区：
文献类型：
--
作者：
Toyonaga K;Miyake Y;Yamasaki S
豚鼠是一种广泛用于研究和开发结核疫苗的动物，因为其病理学疾病过程与人类相似。我们以前曾报道过两种C型凝集素受体，Mincle（巨噬细胞诱导的C型凝集素，也称为Clec 4 e）和MCL（巨噬细胞C型凝集素，也称为Clec 4d），识别分枝杆菌索因子，海藻糖-6，6 ′-二霉菌酸酯（TDM）。在这里，我们的特点的功能的豚鼠同系物Mincle（gpMincle）和MCL（gpMCL）。gpMincle直接与TDM结合，并通过携带ITAM的接头分子FcRγ传递激活信号。而gpMCL缺乏C-末端，不能与TDM结合。在脾脏、淋巴结和腹腔巨噬细胞中检测到gpMincle的mRNA表达，并且在酵母聚糖和TDM刺激下强烈上调。gpMincle的表面表达在活化的巨噬细胞上通过新建立的单克隆抗体检测，所述单克隆抗体也具有阻断活性。该抗体有效抑制BCG感染的巨噬细胞中的TNF产生。总的来说，gpMincle是豚鼠中的TDM受体，并且TDM-Mincle轴参与针对分枝杆菌的宿主免疫应答。
Guinea pig is a widely used animal for research and development of tuberculosis vaccines, since its pathological disease process is similar to that present in humans. We have previously reported that two C-type lectin receptors, Mincle (macrophage inducible C-type lectin, also called Clec4e) and MCL (macrophage C-type lectin, also called Clec4d), recognize the mycobacterial cord factor, trehalose-6,6′-dimycolate (TDM). Here, we characterized the function of the guinea pig homologue of Mincle (gpMincle) and MCL (gpMCL). gpMincle directly bound to TDM and transduced an activating signal through ITAM-bearing adaptor molecule, FcRγ. Whereas, gpMCL lacked C-terminus and failed to bind to TDM. mRNA expression of gpMincle was detected in the spleen, lymph nodes and peritoneal macrophages and it was strongly up-regulated upon stimulation of zymosan and TDM. The surface expression of gpMincle was detected on activated macrophages by a newly established monoclonal antibody that also possesses a blocking activity. This antibody potently suppressed TNF production in BCG-infected macrophages. Collectively, gpMincle is the TDM receptor in the guinea pig and TDM-Mincle axis is involved in host immune responses against mycobacteria.
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影响因子：
4.4
作者：
Botha, T;Ryffel, B
通讯作者：
Ryffel, B
影响因子：
56.9
作者：
Moody, DB;Reinhold, BB;Porcelli, SA
通讯作者：
Porcelli, SA
影响因子：
5.8
作者：
Flynn, JoAnne L.
通讯作者：
Flynn, JoAnne L.
DOI：
10.4049/jimmunol.0904013
发表时间：
2010-03-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Schoenen H;Bodendorfer B;Hitchens K;Manzanero S;Werninghaus K;Nimmerjahn F;Agger EM;Stenger S;Andersen P;Ruland J;Brown GD;Wells C;Lang R
通讯作者：
Lang R
DOI：
10.1073/pnas.0401119101
发表时间：
2004-05-25
影响因子：
11.1
作者：
Ohtsuka, M;Arase, H;Saito, T
通讯作者：
Saito, T