Regulation of differential processing of mouse immunoglobulin mu heavy-chain mRNA.
Regulation of differential processing of mouse immunoglobulin mu heavy-chain mRNA.
复制标题
小鼠免疫球蛋白 mu 重链 mRNA 差异加工的调节。
DOI：
10.1093/nar/15.11.4603
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发表时间：
1987
影响因子：
14.9
通讯作者：
Korn,LJ
中科院分区：
文献类型：
--
作者：
Tsurushita,N;Avdalovic,NM;Korn,LJ
The switch between the synthesis of membrane-bound and secreted IgH during B cell differentiation is accomplished by producing, from a single gene, two alternative forms of μ heavy-chain mRNA that differ only in their 3′ termini. The precursor μ RNA is either polyadenylated at the first poly(A) site, for secreted μ mRNA, or spliced between the C4 and M1 exons, for membrane-bound μ mRNA, in a mutually exclusive manner. To elucidate the molecular mechanism of the differential processing of mouse μ mRNA, we analyzed the expression of various mouse μ gene constructs stably transfected into mouse cell lines. In B cell lines, processing of the exogenously transfected μ gene transcripts accurately reflected the developmental stage of the recipient cells: both secreted and membrane-bound μ mRNAs are produced in early-stage B cells while secreted μ mRNA is primarily produced in late-stage B cells. In fibroblast cell lines, μ mRNAs transcribed from the Moloney murine sarcoma virus LTR promoter were processed primarily to the secreted form. Thus, production of the secreted form seems to be the non-regulated processing pattern. When the splicing signal of the C4-M1 intron was mutagenized, polyadenylation at the first poly(A) site occurred efficiently regardless of the recipient cell lines. On the other hand, when the polyadenylation signal was mutagenized, the splicing occurred efficiently in early-stage B cells, but only weakly in late-stage B cells and fibroblast cells. These results suggest that the splicing of the C4-M1 intron is stimulated in early-stage B cells.
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具有B细胞表面抗原的B细胞杂交瘤的建立。
影响因子：
4.4
作者：
T. Hamano;K. J. Kim;W. Leiserson;R. Asofsky
通讯作者：
R. Asofsky
mu m 和 mu s mRNA 的调节生产需要连接 Poly(A) 添加位点，并且取决于 mu s-mu m 内含子的长度。
DOI：
--
发表时间：
1986
影响因子：
11.1
作者：
M. Peterson;R. Perry
通讯作者：
R. Perry
DOI：
10.1073/pnas.80.3.825
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
OI, VT;MORRISON, SL;BERG, P
通讯作者：
BERG, P
膜结合型和分泌型 IgM 分子的免疫球蛋白 μ 链的 C 末端片段不同
影响因子：
64.5
作者：
M. Kehry;S. Ewald;R. Douglas;C. Sibley;W. Raschke;D. Fambrough;L. Hood
通讯作者：
L. Hood
用于制造分泌特异性抗体的杂交瘤的更好细胞系
影响因子：
64.8
作者：
M. Shulman;C. Wilde;G. Köhler
通讯作者：
G. Köhler