Toxicity of tungsten carbide and cobalt-doped tungsten carbide nanoparticles in mammalian cells in vitro.
Toxicity of tungsten carbide and cobalt-doped tungsten carbide nanoparticles in mammalian cells in vitro.
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体外，哺乳动物细胞中的碳化钨和掺杂钴的碳化钨纳米颗粒的毒性。
DOI：
10.1289/ehp.0800121
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发表时间：
2009-04
影响因子：
10.4
通讯作者：
Schirmer K
中科院分区：
文献类型：
--
作者：
Bastian S;Busch W;Kühnel D;Springer A;Meissner T;Holke R;Scholz S;Iwe M;Pompe W;Gelinsky M;Potthoff A;Richter V;Ikonomidou C;Schirmer K
人们正在探索碳化钨纳米颗粒在硬质合金制造中的应用。为了开发广泛应用的纳米颗粒，应该评估和考虑对人类健康和环境的潜在风险。我们的目标是评估具有良好特性的碳化钨(WC)和掺钴碳化钨(WC-Co)纳米颗粒悬浮液在哺乳动物细胞阵列中的毒性。我们研究了WC和WC-Co(10%重量含量钴)纳米颗粒在不同的人类细胞系(肺、皮肤和结肠)以及大鼠神经元和神经胶质细胞(即原代神经元和星形胶质细胞培养物以及少突胶质细胞前体细胞系OLN-93)中的急性毒性。此外，利用电子显微镜，我们评估了纳米颗粒是否可以被活细胞摄取。我们选择这些体外系统是为了评估纳米颗粒对不同哺乳动物器官(即肺、皮肤、肠道和脑)的潜在毒性。化学物理表征证实，WC和平均粒径为145 nm的WC-Co纳米颗粒在含血清细胞培养液中形成稳定的悬浮液。WC纳米颗粒对所研究的细胞系没有明显的毒性。然而，当颗粒中掺入钴时，细胞毒性变得明显，最敏感的是星形胶质细胞和结肠上皮细胞。根据颗粒中的离子钴含量，WC-Co纳米颗粒的细胞毒性高于预期。电子显微镜分析表明，在哺乳动物细胞中存在WC纳米颗粒。我们的研究结果表明，WC纳米颗粒掺入Co后，其细胞毒性效应显著增强，而WC-Co颗粒的存在是引起这种组合效应的关键。
Tungsten carbide nanoparticles are being explored for their use in the manufacture of hard metals. To develop nanoparticles for broad applications, potential risks to human health and the environment should be evaluated and taken into consideration. We aimed to assess the toxicity of well-characterized tungsten carbide (WC) and cobaltdoped tungsten carbide (WC-Co) nanoparticle suspensions in an array of mammalian cells. We examined acute toxicity of WC and of WC-Co (10% weight content Co) nanoparticles in different human cell lines (lung, skin, and colon) as well as in rat neuronal and glial cells (i.e., primary neuronal and astroglial cultures and the oligodendro cyte precursor cell line OLN-93). Furthermore, using electron microscopy, we assessed whether nanoparticles can be taken up by living cells. We chose these in vitro systems in order to evaluate for potential toxicity of the nanoparticles in different mammalian organs (i.e., lung, skin, intestine, and brain). Chemical–physical characterization confirmed that WC as well as WC-Co nanoparticles with a mean particle size of 145 nm form stable suspensions in serum-containing cell culture media. WC nanoparticles were not acutely toxic to the studied cell lines. However, cytotoxicity became apparent when particles were doped with Co. The most sensitive were astrocytes and colon epithelial cells. Cytotoxicity of WC-Co nanoparticles was higher than expected based on the ionic Co content of the particles. Analysis by electron microscopy demonstrated presence of WC nanoparticles within mammalian cells. Our findings demonstrate that doping of WC nanoparticles with Co markedly increases their cytotoxic effect and that the presence of WC-Co in particulate form is essential to elicit this combinatorial effect.
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DOI：
10.1016/s0921-8777(96)00052-3
发表时间：
1997-01-31
期刊：
MUTATION RESEARCH-DNA REPAIR
影响因子：
--
作者：
Kasten, U;Mullenders, LHF;Hartwig, A
通讯作者：
Hartwig, A
影响因子：
46.9
作者：
Colvin, VL
通讯作者：
Colvin, VL
影响因子：
11.4
作者：
Limbach, Ludwig K.;Wick, Peter;Stark, Wendelin J.
通讯作者：
Stark, Wendelin J.
影响因子：
3.5
作者：
LISON, D;LAUWERYS, R
通讯作者：
LAUWERYS, R
影响因子：
4.2
作者：
Dringen, R;Pawlowski, PG;Hirrlinger, J
通讯作者：
Hirrlinger, J