Perfecting and extending the near-infrared imaging window.
Perfecting and extending the near-infrared imaging window.
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完善和扩展近红外成像窗口。
DOI：
10.1038/s41377-021-00628-0
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发表时间：
2021-09-24
期刊：
影响因子：
--
通讯作者：
Qian J
中科院分区：
文献类型：
--
作者：
Feng Z;Tang T;Wu T;Yu X;Zhang Y;Wang M;Zheng J;Ying Y;Chen S;Zhou J;Fan X;Zhang D;Li S;Zhang M;Qian J
在第二近红外窗口（NIR-II）的活体荧光成像已被认为是一种有前途的技术，用于可视化哺乳动物。但是，NIR-II区域的定义和卓越绩效的解释机制仍有待完善。在此，我们模拟了近红外区域（至2340 nm）中的光子传播，证实了水在活体成像中的适度光吸收的积极贡献，并将NIR-II窗口完善为900-1880 nm，其中1400-1500 nm和1700-1880 nm分别定义为NIR-IIx和NIR-IIc区域。此外，2080-2340 nm被新提出作为第三近红外（NIR-III）窗口，这被认为是提供最好的成像质量。大脑中的宽视野荧光显微镜在NIR-IIx区域周围进行，具有出色的光学切片强度和迄今为止活体NIR-II荧光显微镜的最大成像深度。我们还提出了1400 nm的长通检测在非峰值NIR-II成像的性能超过NIR-IIb成像，使用明亮的荧光团与短发射波长。生物组织的适度光吸收有利于成像性能。第二近红外窗口被完善为900-1880 nm，并且2080-2340 nm被提出作为第三近红外窗口。
In vivo fluorescence imaging in the second near-infrared window (NIR-II) has been considered as a promising technique for visualizing mammals. However, the definition of the NIR-II region and the mechanism accounting for the excellent performance still need to be perfected. Herein, we simulate the photon propagation in the NIR region (to 2340 nm), confirm the positive contribution of moderate light absorption by water in intravital imaging and perfect the NIR-II window as 900–1880 nm, where 1400–1500 and 1700–1880 nm are defined as NIR-IIx and NIR-IIc regions, respectively. Moreover, 2080–2340 nm is newly proposed as the third near-infrared (NIR-III) window, which is believed to provide the best imaging quality. The wide-field fluorescence microscopy in the brain is performed around the NIR-IIx region, with excellent optical sectioning strength and the largest imaging depth of intravital NIR-II fluorescence microscopy to date. We also propose 1400 nm long-pass detection in off-peak NIR-II imaging whose performance exceeds that of NIR-IIb imaging, using bright fluorophores with short emission wavelength. Moderate light absorption by bio-tissue is conducive to the imaging performance. The second near-infrared window is perfected as 900–1880 nm, and 2080–2340 nm is proposed as the third near-infrared window.
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影响因子：
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