Genome-wide association mapping of sodium and potassium concentration in rice grains and shoots under alternate wetting and drying and continuously flooded irrigation.
Genome-wide association mapping of sodium and potassium concentration in rice grains and shoots under alternate wetting and drying and continuously flooded irrigation.
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DOI：
10.1007/s00122-021-03828-9
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发表时间：
2021-07
期刊：
影响因子：
--
通讯作者：
Norton GJ
中科院分区：
文献类型：
--
作者：
Chen C;Travis AJ;Hossain M;Islam MR;Price AH;Norton GJ
在来自aus亚群体的水稻田间生长群体中鉴定大量的钠积累QTL和候选基因。水稻（Oryza sativa L.）是全球重要的谷类作物。钠（Na+）和钾（K+）是影响水稻生长的主要一价离子，探讨它们的吸收机制有助于了解水稻生物学特性。由于Na+和K+的平衡在水稻对盐度的适应中起着重要作用，因此这种生物学可能为寻找耐盐性提供信息。在这项研究中，Na+和K+的浓度和Na+/K+的比例在粮食和芽中进行了分析，在孟加拉和阿萨姆Aus面板生长在田间条件下连续淹没（CF）和干湿交替（AWD）灌溉。总体而言，AWD灌溉显着降低Na+浓度和增加K+浓度的芽和谷物相比，CF下生长的植物。使用有效的混合模型对Na+、K+浓度和Na+/K+比率进行全基因组关联作图，其中具有200万个SNP。仅报告了含有两个以上显著SNP（p < 0.0001）并且这些显著SNP中至少一个超过10%错误发现率的QTL。在所有性状和田间条件下，共检测到106个与Na+浓度和Na+/K+比值相关的QTL，其中48个QTL与多个性状和/或水分条件相关。讨论了4个显著的QTL（1个在第1和11染色体上，2个在第2染色体上）和4个候选基因（OsHKT 1;5、OsNHX 2、LOC_Os02g32490和OsFAD 2_1）的单倍型变异。这些QTL/候选基因可能有助于培育积累低浓度钠的水稻。在线版本包含补充材料，可通过10.1007/s 00122 -021-03828-9获得。
Identification of a large number of QTL and candidate genes for sodium accumulation in a field grown population of rice derived from the aus subpopulation. Rice (Oryza sativa L.) is a globally important cereal crop. Sodium (Na+) and potassium (K+) are the major monovalent ions which affect rice growth, and exploring their uptake mechanisms will be useful for understanding rice biology. Since the balance of Na+ and K+ plays a significant role in adaptation of rice to salinity, that biology might inform the search for tolerance. In this study, the Na+ and K+ concentration and Na+/K+ ratio in grains and shoots were analyzed in the Bengal and Assam Aus Panel grown in field conditions under continuously flooded (CF) and alternate wetting and drying (AWD) irrigation. Overall, AWD irrigation significantly reduced the Na+ concentration and increased the K+ concentration in shoots and grains compared to the plants grown under CF. Genome-wide association mapping was conducted on Na+, K+ concentration and Na+/K+ ratio with 2 million SNPs using an efficient mixed model. Only QTLs which contained more than two significant SNPs (p < 0.0001) and where at least one of these significant SNPs passed a 10% false discovery rate were reported. A total of 106 QTLs were identified as being associated with Na+ concentration and Na+/K+ ratio across all traits and field conditions, with 48 QTLs found in multiple traits and/or water conditions. Four notable QTLs (one each on chromosomes 1 and 11, two on chromosome 2) and the haplotype variants of four candidate genes (OsHKT1;5, OsNHX2, LOC_Os02g32490 and OsFAD2_1) are discussed. The QTLs/candidate genes identified here could be useful for breeding rice that accumulates lower concentrations of sodium. The online version contains supplementary material available at 10.1007/s00122-021-03828-9.
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DOI：
10.1186/s12284-016-0119-0
发表时间：
2016-12
期刊：
Rice (New York, N.Y.)
影响因子：
--
作者：
Kim H;Jung J;Singh N;Greenberg A;Doyle JJ;Tyagi W;Chung JW;Kimball J;Hamilton RS;McCouch SR
通讯作者：
McCouch SR
影响因子：
7.4
作者：
Koyama, ML;Levesley, A;Yeo, AR
通讯作者：
Yeo, AR
影响因子：
2
作者：
An, Hongzhou;Liu, Kai;Wan, Jianmin
通讯作者：
Wan, Jianmin
影响因子：
5.1
作者：
Khush, GS
通讯作者：
Khush, GS
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y