Polymer Modified Carbon Fiber Microelectrodes for Precision Neurotransmitter Metabolite Measurements
Polymer Modified Carbon Fiber Microelectrodes for Precision Neurotransmitter Metabolite Measurements
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用于精确神经递质代谢物测量的聚合物改性碳纤维微电极
DOI：
10.1149/1945-7111/abcb6d
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发表时间：
2020
影响因子：
3.9
通讯作者：
Zestos, Alexander G.
中科院分区：
文献类型：
--
作者：
Wonnenberg, Pauline;Cho, Whirang;Liu, Favian;Asrat, Thomas;Zestos, Alexander G.
碳纤维微电极被认为是检测多巴胺等神经递质的标准电极之一。DA对许多药理和行为状态具有重要的生理意义，但很容易在快速、亚秒的时间尺度上代谢。近年来，多巴胺代谢产物3-甲氧基酪胺(3-MT)和3，4-二羟基苯乙醛(DOPAL)被发现参与运动控制和进行性神经元变性等生理功能。然而，目前还没有检测和区分它们与DA的方法。在这项研究中，我们展示了类似结构的神经化学物质，如DA，3-MT和DOPAL的联合检测。我们通过用聚乙烯亚胺(PEI)和聚(3，4-乙二氧基噻吩基)聚苯乙烯磺酸盐(PEDOT：PSS)聚合物电沉积CFME来实现这一点。这赋予了裸露的未经修饰的CFME表面电荷、物理和化学上的差异，从而提高了神经递质检测的灵敏度和选择性。3-MT、DOPAL和DA的鉴别和检测将有助于进一步了解这些多巴胺能代谢产物在体内发挥的重要生理作用。
Carbon fiber-microelectrodes (CFMEs) are considered to be one of the standard electrodes for neurotransmitter detection such as dopamine (DA). DA is physiologically important for many pharmacological and behavioral states, but is readily metabolized on a fast, subsecond timescale. Recently, DA metabolites such as 3-methoxytyramine (3-MT) and 3, 4-dihydroxyphenylacetaldehyde (DOPAL) were found to be involved in physiological functions, such as movement control and progressive neuro degeneration. However, there is no current assay to detect and differentiate them from DA. In this study, we demonstrate the co-detection of similarly structured neurochemicals such as DA, 3-MT, and DOPAL. We accomplished this through electrodepositing CFMEs with polyethyleneimine (PEI) and poly (3, 4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT: PSS) polymers. This endowed the bare unmodified CFMEs with surface charge, physical, and chemical differences, which resulted in the improved sensitivity and selectivity of neurotransmitter detection. The differentiation and detection of 3-MT, DOPAL, and DA will potentially help further understand the important physiological roles that these dopaminergic metabolites play in vivo.
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黑质纹状体、中脑边缘和中皮质神经元体内多巴胺的释放：3-甲氧基酪胺测量的效用。
影响因子：
21.1
作者：
P. Wood;C. Altar
通讯作者：
C. Altar
影响因子：
3.9
作者：
Cho, Whirang;Liu, Favian;Zestos, Alexander G.
通讯作者：
Zestos, Alexander G.
影响因子：
3.9
作者：
Mendoza, Alexander;Asrat, Thomas;Zestos, Alexander G.
通讯作者：
Zestos, Alexander G.
影响因子：
7.4
作者：
Bath, BD;Michael, DJ;Wightman, RM
通讯作者：
Wightman, RM
DOI：
10.1016/j.snb.2013.03.066
发表时间：
2013-06
期刊：
Sensors and actuators. B, Chemical
影响因子：
--
作者：
Zestos AG;Nguyen MD;Poe BL;Jacobs CB;Venton BJ
通讯作者：
Venton BJ