In silico reconstitution of DNA replication. Lessons from single-molecule imaging and cryo-tomography applied to single-particle cryo-EM.
In silico reconstitution of DNA replication. Lessons from single-molecule imaging and cryo-tomography applied to single-particle cryo-EM.
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DOI：
10.1016/j.sbi.2021.11.015
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发表时间：
2022-03
影响因子：
6.8
通讯作者：
Costa A
中科院分区：
文献类型：
--
作者：
Greiwe JF;Zanetti G;Miller TCR;Costa A
DNA复制已经在体外与酵母蛋白重组，最小系统需要16个不同复制因子的协调组装，由42个多肽组成。为了在单一残基水平上了解这些因素之间的分子相互作用，正在开发新的结构生物学工具。受到单分子荧光成像和冷冻层析成像技术的启发，人们利用新颖的单粒子低温电磁实验来表征复制解旋酶的负载结构机制。在这里，我们讨论了单粒子低温电磁数据的电子重建如何能够帮助描述用传统三维分类工具难以接近的动态系统。
DNA replication has been reconstituted in vitro with yeast proteins, and the minimal system requires the coordinated assembly of 16 distinct replication factors, consisting of 42 polypeptides. To understand the molecular interplay between these factors at the single residue level, new structural biology tools are being developed. Inspired by advances in single-molecule fluorescence imaging and cryo-tomography, novel single-particle cryo-EM experiments have been used to characterise the structural mechanism for the loading of the replicative helicase. Here, we discuss how in silico reconstitution of single-particle cryo-EM data can help describe dynamic systems that are difficult to approach with conventional three-dimensional classification tools.
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影响因子：
16.6
作者：
Hutchings J;Stancheva VG;Brown NR;Cheung ACM;Miller EA;Zanetti G
通讯作者：
Zanetti G
影响因子：
16.6
作者：
Hutchings J;Stancheva V;Miller EA;Zanetti G
通讯作者：
Zanetti G
影响因子：
64.8
作者：
Ke, Zunlong;Oton, Joaquin;Briggs, John A. G.
通讯作者：
Briggs, John A. G.
影响因子：
56.9
作者：
MARAHRENS, Y;STILLMAN, B
通讯作者：
STILLMAN, B
DOI：
10.1073/pnas.0911500106
发表时间：
2009-12-01
影响因子：
11.1
作者：
Evrin, Cecile;Clarke, Pippa;Speck, Christian
通讯作者：
Speck, Christian