A non-invasive method of quantifying pancreatic volume in mice using micro-MRI.
A non-invasive method of quantifying pancreatic volume in mice using micro-MRI.
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DOI：
10.1371/journal.pone.0092263
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Husain SZ
中科院分区：
文献类型：
--
作者：
Paredes JL;Orabi AI;Ahmad T;Benbourenane I;Tobita K;Tadros S;Bae KT;Husain SZ
In experimental models of pancreatic growth and recovery, changes in pancreatic size are assessed by euthanizing a large cohort of animals at varying time points and measuring organ mass. However, to ascertain this information in clinical practice, patients with pancreatic disorders routinely undergo non-invasive cross-sectional imaging of the pancreas using magnetic resonance imaging (MRI) or computed tomography (CT). The aim of the current study was to develop a thin-sliced, optimized sequence protocol using a high field MRI to accurately calculate pancreatic volumes in the most common experimental animal, the mouse. Using a 7 Telsa Bruker micro-MRI system, we performed abdominal imaging in whole-fixed mice in three standard planes: axial, sagittal, and coronal. The contour of the pancreas was traced using Vitrea software and then transformed into a 3-dimensional (3D) reconstruction, from which volumetric measurements were calculated. Images were optimized using heart perfusion-fixation, T1 sequence analysis, and 0.2 to 0.4 mm thick slices. As proof of principle, increases in pancreatic volume among mice of different ages correlated tightly with increasing body weight. In summary, this is the first study to measure pancreatic volumes in mice, using a high field 7 Tesla micro-MRI and a thin-sliced, optimized sequence protocol. We anticipate that micro-MRI will improve the ability to non-invasively quantify changes in pancreatic size and will dramatically reduce the number of animals required to serially assess pancreatic growth and recovery.
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影响因子：
--
作者：
Geraghty, EM;Boone, JM;Jain, K
通讯作者：
Jain, K
影响因子：
6.4
作者：
Grimm, J;Potthast, A;Moore, A
通讯作者：
Moore, A
影响因子：
1.2
作者：
Orabi, Abrahim I.;Muili, Kamaldeen A.;Husain, Sohail Z.
通讯作者：
Husain, Sohail Z.
DOI：
10.3791/3564
发表时间：
2012-07-30
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Gage, Gregory J;Kipke, Daryl R;Shain, William
通讯作者：
Shain, William
影响因子：
2.1
作者：
Attili, Anil K.;Schuster, Andreas;Nagel, Eike;Reiber, Johan H. C.;van der Geest, Rob J.
通讯作者：
van der Geest, Rob J.