New insights into the ultrasound impact on covalent reactions of myofibrillar protein.
New insights into the ultrasound impact on covalent reactions of myofibrillar protein.
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DOI：
10.1016/j.ultsonch.2022.105973
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发表时间：
2022-03
影响因子：
8.4
通讯作者：
Xu X
中科院分区：
文献类型：
--
作者：
Chen J;Chen X;Zhou G;Xu X
研究了超声对肌原纤维蛋白共价反应的影响。超声加速了肌原纤维蛋白的反应。超声与自由基氧化联合治疗效果更好。F2基团的独特协同作用不能简单地归因于过氧化氢的解离作用。本研究选择两种不同的共价反应，即碱性反应和自由基氧化反应，比较超声治疗（UDT）强化效果的差异。通过蛋白电泳和结合没食子酸（GA）测定验证了接枝效果。此外，肌纤维蛋白（MPN）聚集体之间的非共价相互作用被UDT破坏，证明了这一点，即更小的颗粒大小和更高的ζ-电位。相比之下，三级结构指数和圆二色性的结果表明，udt辅助自由基氧化可以产生更好的共轭物和更高的结构性能。原子力显微镜（AFME）和蛋白质柔韧性分析表明，碱性反应后mpn呈不规则球形，而自由基氧化过程中mpn仍保持纤维状结构。由此可见，UDT与自由基氧化的结合更有效地增强了声空化对mpn的影响，其机制是粘度特性、微观结构和声空化自由基的变化。
The effects of ultrasound on covalent reactions of myofibrillar protein were investigated. Ultrasound accelerated the reactions of myofibrillar protein. The combination of ultrasound and free radical oxidation was more effective. The distinctive synergy of the F2 group cannot be simply attributed to the dissociation of hydrogen peroxide. In this work, two different covalent reactions, namely, alkaline reaction and free radical oxidation, were selected to compare the difference in the strengthening effects of ultrasound treatment (UDT). The grafting effects were verified by protein electrophoresis and bound gallic acid (GA) assay. Furthermore, non-covalent interactions between myofibrillar protein (MPN) aggregates were destroyed by UDT, as proved by the lower particle sizes and higher ζ-potential. Comparatively, the results from tertiary structure index and circular dichroism revealed UDT-assisted free radical oxidation could lead to better conjugates with greater structural properties. The atomic force microscope (AFME) and protein flexibility showed that MPNs appeared to display as irregular spherical particles after alkaline reaction, however, maintained fibrous structure during the free radical oxidation. Consequently, the combination of UDT and free radical oxidation were more effectively for strengthening the influence of acoustic cavitation on MPNs, of which mechanism was the changes in viscosity properties, microstructure and acoustic cavitation radicals.
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影响因子：
8.4
作者：
Chen, Jiahui;Zhang, Xing;Xu, Xinglian
通讯作者：
Xu, Xinglian
影响因子：
8.4
作者：
Liu, Haotian;Zhang, Huan;Kong, Baohua
通讯作者：
Kong, Baohua
DOI：
10.1016/j.ijbiomac.2017.12.167
发表时间：
2018-05-01
影响因子：
8.2
作者：
Amiri, Amir;Sharifian, Parisa;Soltanizadeh, Nafiseh
通讯作者：
Soltanizadeh, Nafiseh
DOI：
10.1016/j.ifset.2019.102191
发表时间：
2019-08-01
影响因子：
6.6
作者：
Amiri, Amir;Mousakhani-Ganjeh, Alireza;Kenari, Reza Esmaeilzadeh
通讯作者：
Kenari, Reza Esmaeilzadeh
影响因子：
6
作者：
Chen, Jiahui;Xu, Yujuan;Xu, Xinglian
通讯作者：
Xu, Xinglian