Diffusiophoretic Particle Penetration into Bacterial Biofilms.
Diffusiophoretic Particle Penetration into Bacterial Biofilms.
复制标题
扩散电泳颗粒对细菌生物膜的渗透。
DOI：
10.1021/acsami.3c03190
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发表时间：
2023-07-19
影响因子：
9.5
通讯作者：
Stone, Howard A.
中科院分区：
文献类型：
--
作者：
Somasundar, Ambika;Qin, Boyang;Shim, Suin;Bassler, Bonnie L.;Stone, Howard A.
细菌生物膜是附着在表面的细胞群落。这些群落代表了地球上细菌生命的主要形式。生物膜的一个定义特征是三维细胞外聚合物基质，其通过充当化学品（如抗微生物剂）渗透的机械屏障来保护驻留细胞。除了对抗生素治疗的排斥外，生物膜还非常难以从表面去除。一种有希望的但相对未充分探索的生物膜控制方法是通过使颗粒能够穿透来破坏细胞外聚合物基质，以增加生物膜对抗菌剂的敏感性。在这项工作中，我们研究了外部施加的化学梯度作为一种机制，运输聚苯乙烯颗粒进入细菌生物膜。我们表明，使用去离子（DI）水的预洗步骤的预处理的生物膜是必不可少的改变生物膜，所以它采取了由电解质产生的进一步的化学梯度的应用程序的微米和纳米粒子。使用不同的颗粒和化学品，我们记录的运输行为，导致颗粒运动进入生物膜和其进一步逆转的生物膜。我们的研究结果表明，在拥挤的大分子环境中，化学梯度在破坏生物膜基质和调节颗粒运输中的重要性，并建议在其他生理系统中的颗粒运输和递送的潜在应用。
Bacterial biofilms are communities of cells adhered to surfaces. These communities represent a predominant form of bacterial life on Earth. A defining feature of a biofilm is the three-dimensional extracellular polymer matrix that protects resident cells by acting as a mechanical barrier to the penetration of chemicals, such as antimicrobials. Beyond being recalcitrant to antibiotic treatment, biofilms are notoriously difficult to remove from surfaces. A promising, but relatively underexplored, approach to biofilm control is to disrupt the extracellular polymer matrix by enabling penetration of particles to increase the susceptibility of biofilms to antimicrobials. In this work, we investigate externally imposed chemical gradients as a mechanism to transport polystyrene particles into bacterial biofilms. We show that preconditioning the biofilm with a prewash step using deionized (DI) water is essential for altering the biofilm so it takes up the micro- and nanoparticles by the application of a further chemical gradient created by an electrolyte. Using different particles and chemicals, we document the transport behavior that leads to particle motion into the biofilm and its further reversal out of the biofilm. Our results demonstrate the importance of chemical gradients in disrupting the biofilm matrix and regulating particle transport in crowded macromolecular environments, and suggest potential applications of particle transport and delivery in other physiological systems.
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DOI：
10.1073/pnas.1611494113
发表时间：
2016-09-06
影响因子：
11.1
作者：
Yan, Jing;Sharo, Andrew G.;Bassler, Bonnie L.
通讯作者：
Bassler, Bonnie L.
影响因子：
28.3
作者：
Kim, Minyoung Kevin;Ingremeau, Francois;Zhao, Aishan;Bassler, Bonnie L.;Stone, Howard A.
通讯作者：
Stone, Howard A.
细胞外矩阵介导的渗透压驱动弧菌霍乱生物膜膨胀和作弊者排除。
影响因子：
16.6
作者：
Yan J;Nadell CD;Stone HA;Wingreen NS;Bassler BL
通讯作者：
Bassler BL
DOI：
10.1126/science.1222981
发表时间：
2012-07-13
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Berk V;Fong JC;Dempsey GT;Develioglu ON;Zhuang X;Liphardt J;Yildiz FH;Chu S
通讯作者：
Chu S
影响因子：
14
作者：
Cao, Bing;Lyu, Xiaoming;Hu, Xianglong
通讯作者：
Hu, Xianglong