Size-dependent protein segregation at membrane interfaces.
Size-dependent protein segregation at membrane interfaces.
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DOI：
10.1038/nphys3678
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发表时间：
2016-07
期刊：
影响因子：
19.6
通讯作者：
中科院分区：
文献类型：
--
作者：
在细胞-细胞连接处形成的膜界面与膜蛋白组织的特征模式有关，例如上皮连接复合物中E-cadherin的富集和免疫突触信号中心中CD45的排除。为了分离蛋白质大小在这些过程中的作用，我们在体外用合成结合蛋白和非结合蛋白修饰的巨大单层囊泡重建了膜界面。我们发现，结合蛋白和非结合蛋白之间的大小差异可以在细胞骨架没有活性贡献的情况下显著改变它们在膜界面上的组织，非结合蛋白的大小仅增加约5纳米就会导致其从界面上被排除。结合体外测量和蒙特卡罗模拟，我们发现非结合蛋白排斥也受到横向拥挤、结合蛋白亲和力和热驱动的膜高度波动的影响，这些波动会暂时限制进入界面。这种简单、灵敏、高效的被动分离蛋白质的方法对细胞-细胞连接处的信号传导和膜结合细胞器之间的细胞内接触点的蛋白质分选具有重要意义。
Membrane interfaces formed at cell-cell junctions are associated with characteristic patterns of membrane protein organization, such as E-cadherin enrichment in epithelial junctional complexes and CD45 exclusion from the signaling foci of immunological synapses. To isolate the role of protein size in these processes, we reconstituted membrane interfaces in vitro using giant unilamellar vesicles decorated with synthetic binding and non-binding proteins. We show that size differences between binding and non-binding proteins can dramatically alter their organization at membrane interfaces in the absence of active contributions from the cytoskeleton, with as little as a ~5 nm increase in non-binding protein size driving its exclusion from the interface. Combining in vitro measurements with Monte Carlo simulations, we find that non-binding protein exclusion is also influenced by lateral crowding, binding protein affinity, and thermally-driven membrane height fluctuations that transiently limit access to the interface. This simple, sensitive, and highly effective means of passively segregating proteins has implications for signaling at cell-cell junctions and protein sorting at intracellular contact points between membrane-bound organelles.
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DOI：
10.1186/ar559
发表时间：
2002
期刊：
Arthritis research
影响因子：
--
作者：
Dustin ML
通讯作者：
Dustin ML
影响因子：
3.7
作者：
Krobath H;Różycki B;Lipowsky R;Weikl TR
通讯作者：
Weikl TR
影响因子：
20.3
作者：
Cordoba, Shaun-Paul;Choudhuri, Kaushik;van der Merwe, P. Anton
通讯作者：
van der Merwe, P. Anton
影响因子：
7.5
作者：
Kornmann, Benoit
通讯作者：
Kornmann, Benoit
影响因子：
64.8
作者：
Monks, CRF;Freiberg, BA;Kupfer, A
通讯作者：
Kupfer, A