Metabolic Contributions of an Alphaproteobacterial Endosymbiont in the Apicomplexan Cardiosporidium cionae.
Metabolic Contributions of an Alphaproteobacterial Endosymbiont in the Apicomplexan Cardiosporidium cionae.
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DOI：
10.3389/fmicb.2020.580719
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发表时间：
2020
影响因子：
5.2
通讯作者：
Lane CE
中科院分区：
文献类型：
--
作者：
Hunter ES;Paight C;Lane CE
顶复虫是一种多样的原原虫门，几乎完全由感染后生动物的寄生虫组成，包括疟疾、隐孢子虫病和弓形虫病的病原体。2010年，一种单一的顶复合体属肾真菌被描述为其被囊寄主的共生伙伴。在这里，我们提供了寄生姐妹物种的基因组和转录组数据，这些物种是这种互惠共生体，cionae心孢子虫及其相关的细菌内共生体。cionae心孢子虫和肾真菌都能感染被囊宿主，定位于宿主体内相似的器官，并维持细菌内共生。虽然已知许多其他原生生物含有细菌内共生体，但在肾霉菌科分支之外的顶复合体中，这些关联是完全未知的。我们的数据表明，自肾真菌和心孢子虫分化以来，每个谱系中都保留了垂直传播的α-变形菌。这种α-变形菌内共生体的代谢能力高度降低，但为金银花提供必需氨基酸赖氨酸和必需辅因子硫辛酸。这种伙伴关系可能会减少与被囊寄主的资源竞争。然而，我们的数据表明，与肾真菌系统中存在的三个内共生体类群相比，单个α-变形菌内共生体在C. cionae中的贡献是最小的，这可能解释了这些谱系之间的毒力差异。
Apicomplexa is a diverse protistan phylum composed almost exclusively of metazoan-infecting parasites, including the causative agents of malaria, cryptosporidiosis, and toxoplasmosis. A single apicomplexan genus, Nephromyces, was described in 2010 as a mutualist partner to its tunicate host. Here we present genomic and transcriptomic data from the parasitic sister species to this mutualist, Cardiosporidium cionae, and its associated bacterial endosymbiont. Cardiosporidium cionae and Nephromyces both infect tunicate hosts, localize to similar organs within these hosts, and maintain bacterial endosymbionts. Though many other protists are known to harbor bacterial endosymbionts, these associations are completely unknown in Apicomplexa outside of the Nephromycidae clade. Our data indicate that a vertically transmitted α-proteobacteria has been retained in each lineage since Nephromyces and Cardiosporidium diverged. This α-proteobacterial endosymbiont has highly reduced metabolic capabilities, but contributes the essential amino acid lysine, and essential cofactor lipoic acid to C. cionae. This partnership likely reduces resource competition with the tunicate host. However, our data indicate that the contribution of the single α-proteobacterial endosymbiont in C. cionae is minimal compared to the three taxa of endosymbionts present in the Nephromyces system, and is a potential explanation for the virulence disparity between these lineages.
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影响因子：
2.7
作者：
Garrison NL;Rodriguez J;Agnarsson I;Coddington JA;Griswold CE;Hamilton CA;Hedin M;Kocot KM;Ledford JM;Bond JE
通讯作者：
Bond JE
影响因子：
4
作者：
Gubbels MJ;Duraisingh MT
通讯作者：
Duraisingh MT
影响因子：
2.4
作者：
Alarcon, M. E.;Jara-F, A.;Slamovits, C. H.
通讯作者：
Slamovits, C. H.
影响因子：
11.4
作者：
Crawford, Michael J.;Thomsen-Zieger, Nadine;Seeber, Frank
通讯作者：
Seeber, Frank
影响因子：
--
作者：
Blume, Martin;Seeber, Frank
通讯作者：
Seeber, Frank