Three-dimensional three-component particle velocimetry for microscale flows using volumetric scanning
Three-dimensional three-component particle velocimetry for microscale flows using volumetric scanning
复制标题
使用体积扫描进行微尺度流动的三维三分量粒子测速
DOI：
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
J. Posner
中科院分区：
文献类型：
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作者：
S. A. Klein;J. Moran;D. Frakes;J. Posner
我们提出了一个用于测量微观体积中三维三分量 (3D3C) 速度场的诊断平台。成像系统使用高速 Nipkow 转盘共焦显微镜。共焦显微镜使用针孔空间过滤提供光学切片，该过滤可以拒绝来自失焦物体的光。该系统通过使用压电物镜定位器快速平移高数值孔径物镜来完成体积扫描。使用 3D3C 超分辨率粒子成像测速技术对荧光微球的运动进行量化，所有三个维度的瞬时空间分辨率均为 5 µm 或更小。我们以 30 Hz 的 3D 采集速率检查了具有扩展截面的 PDMS 微通道中的 3D3C 流动，并发现与计算模型非常一致。适用于扫描物镜的 PIV 和 PTV 文献中的方程提供了最大可测量速度的估计。该技术允许 3D 粒子运动的等值面可视化和强大的高空间分辨率速度测量，无需校准步骤或重建算法。
We present a diagnostic platform for measuring three-dimensional three-component (3D3C) velocity fields in microscopic volumes. The imaging system uses high-speed Nipkow spinning disk confocal microscopy. Confocal microscopy provides optical sectioning using pinhole spatial filtering which rejects light originating from out-of-focus objects. The system accomplishes volumetric scanning by rapid translation of the high numerical aperture objective using a piezo objective positioner. The motion of fluorescent microspheres is quantified using 3D3C super resolution particle-imaging velocimetry with instantaneous spatial resolutions of the order of 5 µm or less in all three dimensions. We examine 3D3C flow in a PDMS microchannel with an expanding section at 3D acquisition rates of 30 Hz, and find strong agreement with a computational model. Equations from the PIV and PTV literature adapted for a scanning objective provide estimates of maximum measurable velocity. The technique allows for isosurface visualization of 3D particle motion and robust high spatial resolution velocity measurements without requiring a calibration step or reconstruction algorithms.
影响因子：
7.4
作者：
Anderson, JR;Chiu, DT;Whitesides, GM
通讯作者：
Whitesides, GM
影响因子：
5.5
作者：
Schultz KM;Baldwin AD;Kiick KL;Furst EM
通讯作者：
Furst EM