Activation of cytokines and NF-kappa B in corneal epithelial cells infected by respiratory syncytial virus: potential relevance in ocular inflammation and respiratory infection.
Activation of cytokines and NF-kappa B in corneal epithelial cells infected by respiratory syncytial virus: potential relevance in ocular inflammation and respiratory infection.
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DOI：
10.1186/1471-2180-4-28
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发表时间：
2004-07-15
期刊：
影响因子：
4.2
通讯作者：
Barik S
中科院分区：
文献类型：
--
作者：
Bitko V;Garmon NE;Cao T;Estrada B;Oakes JE;Lausch RN;Barik S
呼吸道合胞病毒（RSV）是下呼吸道感染的主要原因，每年夺去数百万人的生命。该病毒感染呼吸道的各种细胞以及驻留的炎性细胞如巨噬细胞。感染激活多种细胞因子，如细胞因子和促炎转录因子NF-κ B，所有这些都是呼吸道疾病的重要参与者。然而，RSV感染的确切自然途径及其病原学仍然相对未知。在本文中，我们测试的假设，人角膜上皮细胞，构成角膜的最外层，可以感染RSV，感染导致激活的促炎大分子。从患有急性呼吸道疾病的儿科患者获得的角膜拭子被发现以高频率含有RSV（72个样品中的43个阳性，即，60%）。组织培养中的原代角膜上皮细胞支持RSV的稳健感染和生产性生长。感染导致TNF-α、IL-6和16种趋化因子以及NF-κB的活化。三种促炎性CXC趋化因子（CXC，I-TAC，IP-10）经历最大激活。眼上皮容易被RSV感染。促炎细胞因子可能在儿科呼吸道感染患者常见的炎症和结膜炎的病因学中起关键作用。RSV-眼相互作用在RSV传播、RSV疾病的免疫病理学和结膜炎的管理中具有重要意义。
Respiratory syncytial virus (RSV) is a major cause of lower respiratory tract infection, claiming millions of lives annually. The virus infects various cells of the respiratory tract as well as resident inflammatory cells such as macrophages. Infection activates a variety of cellular factors such as cytokines and the pro-inflammatory transcription factor, NF-kappa B, all of which are important players in the respiratory disease. However, the exact natural route of RSV infection and its etiology remain relatively unknown. In this paper, we test the hypothesis that human corneal epithelial cells, which constitute the outermost layer of the cornea, can be infected with RSV, and that the infection leads to the activation of proinflammatory macromolecules. Corneal swabs obtained from pediatric patients with acute respiratory disease were found to contain RSV at a high frequency (43 positive out of 72 samples, i.e., 60%). Primary corneal epithelial cells in tissue culture supported robust infection and productive growth of RSV. Infection resulted in the activation of TNF-α, IL-6 and sixteen chemokines as well as NF-κB. Three proinflammatory CXC chemokines (MIG, I-TAC, IP-10) underwent the greatest activation. The ocular epithelium is readily infected by RSV. The pro-inflammatory cytokines are likely to play critical roles in the etiology of inflammation and conjunctivitis commonly seen in pediatric patients with respiratory infections. RSV-eye interactions have important implications in RSV transmission, immunopathology of RSV disease, and in the management of conjunctivitis.
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