New methods for chicken embryo manipulations.
New methods for chicken embryo manipulations.
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DOI：
10.1002/jemt.20753
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发表时间：
2010-01
影响因子：
2.5
通讯作者：
De Bellard, Maria Elena
中科院分区：
文献类型：
--
作者：
El-Ghali, Nissrine;Rabadi, Maes;Ezin, Akouavi M.;De Bellard, Maria Elena
成像一个生长中的胚胎，同时研究特定分子的发育功能的能力为胚胎发生提供了宝贵的信息。然而，直到最近，这种方法很难实现，因为需要先进的技术，也因为没有一种简单的方法来减少对胚胎的损害。在这里，我们提出了一种新的方法，使整个鸡胚胎的EC培养适应于延时成像和使用阻断剂的功能分子研究。我们的方法的新颖性源于在卵外和卵内应用阻断剂的能力。我们能够通过在含有这些分子的组织培养基中培养出卵的发育中的胚胎，或者将它们注射到卵中的活胚胎下面，来研究一组分子的功能。卵内制备特别有价值，因为它延长了研究分子发育功能的时间，并为筛选一批分子提供了一种简单、可重复的方法。这些新技术将被证明对可视化和理解特定分子在胚胎形态发生（包括血管形成）中的作用非常有帮助。
The capacity to image a growing embryo while simultaneously studying the developmental function of specific molecules provides invaluable information on embryogenesis. However, until recently, this approach was accomplished with difficulty both because of the advanced technology needed and because an easy method of minimizing damage to the embryo was unavailable. Here we present a novel way of adapting the well-known EC culture of whole chick embryos to time-lapse imaging and to functional molecular studies using blocking agents. The novelty of our method stems from the ability to apply blocking agents ex ovo as well as in ovo. We were able to study the function of a set of molecules by culturing developing embryos ex ovo in tissue culture media containing these molecules or by injecting them underneath the live embryo in ovo. The in ovo preparation is particularly valuable since it extends the period of time during which the developmental function of the molecule can be studied and it provides an easy, reproducible method for screening a batch of molecules. These new techniques will prove very helpful in visualizing and understanding the role of specific molecules during embryonic morphogenesis, including blood vessel formation.
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DOI：
10.1007/bf00309751
发表时间：
1987-01-01
期刊：
ANATOMY AND EMBRYOLOGY
影响因子：
--
作者：
FLAMME, I
通讯作者：
FLAMME, I
影响因子：
2.7
作者：
Giamario, C;Petitte, JN;Mozdziak, PE
通讯作者：
Mozdziak, PE
影响因子：
3.7
作者：
Boyd WA;McBride SJ;Freedman JH
通讯作者：
Freedman JH
影响因子：
56.9
作者：
Kulesa, PM;Fraser, SE
通讯作者：
Fraser, SE
影响因子：
4.5
作者：
Shao, Yu;Chan, Chi Yu;Ding, Ye
通讯作者：
Ding, Ye