A whole-genome shotgun approach for assembling and anchoring the hexaploid bread wheat genome.
A whole-genome shotgun approach for assembling and anchoring the hexaploid bread wheat genome.
复制标题
用于组装和锚定六倍体面包小麦基因组的全基因组鸟枪方法。
DOI:
10.1186/s13059-015-0582-8
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发表时间:
2015-01-31
期刊:
影响因子:
12.3
通讯作者:
Rokhsar DS
中科院分区:
文献类型:
--
作者:
Chapman JA;Mascher M;Buluç A;Barry K;Georganas E;Session A;Strnadova V;Jenkins J;Sehgal S;Oliker L;Schmutz J;Yelick KA;Scholz U;Waugh R;Poland JA;Muehlbauer GJ;Stein N;Rokhsar DS
Polyploid species have long been thought to be recalcitrant to whole-genome assembly. By combining high-throughput sequencing, recent developments in parallel computing, and genetic mapping, we derive, de novo, a sequence assembly representing 9.1 Gbp of the highly repetitive 16 Gbp genome of hexaploid wheat, Triticum aestivum, and assign 7.1 Gb of this assembly to chromosomal locations. The genome representation and accuracy of our assembly is comparable or even exceeds that of a chromosome-by-chromosome shotgun assembly. Our assembly and mapping strategy uses only short read sequencing technology and is applicable to any species where it is possible to construct a mapping population. The online version of this article (doi:10.1186/s13059-015-0582-8) contains supplementary material, which is available to authorized users.
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影响因子:
3.7
作者:
Chapman JA;Ho I;Sunkara S;Luo S;Schroth GP;Rokhsar DS
通讯作者:
Rokhsar DS
影响因子:
1.7
作者:
Jurka, J;Kapitonov, VV;Walichiewicz, J
通讯作者:
Walichiewicz, J
影响因子:
5.8
作者:
Li, Heng
通讯作者:
Li, Heng
影响因子:
7
作者:
Green, P
通讯作者:
Green, P
DOI:
10.1093/bioinformatics/btt178
发表时间:
2013-06-15
期刊:
Bioinformatics (Oxford, England)
影响因子:
--
作者:
Birol I;Raymond A;Jackman SD;Pleasance S;Coope R;Taylor GA;Yuen MM;Keeling CI;Brand D;Vandervalk BP;Kirk H;Pandoh P;Moore RA;Zhao Y;Mungall AJ;Jaquish B;Yanchuk A;Ritland C;Boyle B;Bousquet J;Ritland K;Mackay J;Bohlmann J;Jones SJ
通讯作者:
Jones SJ