Rs1800625 in the receptor for advanced glycation end products gene predisposes to sepsis and multiple organ dysfunction syndrome in patients with major trauma.
Rs1800625 in the receptor for advanced glycation end products gene predisposes to sepsis and multiple organ dysfunction syndrome in patients with major trauma.
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晚期糖基化终末产物基因受体中的Rs1800625易导致重大创伤患者败血症和多器官功能障碍综合征
DOI：
10.1186/s13054-014-0727-2
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发表时间：
2015-01-09
期刊：
影响因子：
--
通讯作者：
Jiang JX
中科院分区：
文献类型：
--
作者：
Zeng L;Du J;Gu W;Zhang AQ;Wang HY;Wen DL;Qiu L;Yang XT;Sun JH;Zhang M;Hao J;Jiang JX
晚期糖基化终末产物受体（receptor for advanced glycation end products，RECEPTOR）是免疫球蛋白超家族中的一种跨膜受体，在脓毒症的发病机制中发挥重要作用。我们之前的研究表明，rs 1800625（− 429 T/C）与严重创伤患者的脓毒症发病率和多器官功能障碍综合征（MODS）有很强的临床相关性。在这项研究中，我们扩大了样本量，增加了两个验证人群，并检查了不同基因型的受试者的外周血白细胞表面的LPS刺激的表达。采用焦磷酸测序法对重庆地区837例汉族严重创伤患者进行了Rs 1800625基因分型。然后，我们在340名浙江和347名云南患者中验证了临床相关性。流式细胞术检测外周血单个核细胞表面CD 45的表达。结果表明，rs 1800625与重庆、浙江、云南地区严重创伤患者脓毒症发病率和MODS发生率显著相关。CC基因型患者脓毒症发生率和严重创伤后MODS发生率较低。CC基因型患者中，CD 45表达显著高于CC基因型患者（P = 0.009）。rs 1800625多态性是一种功能性单核苷酸多态性，可使严重创伤患者对脓毒症和MODS易感。
The receptor for advanced glycation end products (RAGE) is a transmembrane receptor of the immunoglobulin superfamily, it plays pivotal roles in the pathogenesis of sepsis in several ways. Our previous study showed that rs1800625 (−429T/C) revealed a strong clinical relevance with sepsis morbidity rate and multiple organ dysfunction syndrome (MODS) in patients with major trauma. In this study, we enlarged the sample size, added two validation populations and examined the expression of RAGE on the surface of peripheral leukocytes to ex vivo lipopolysaccharide (LPS) stimulation in subjects with different genotypes. Rs1800625 was genotyped using pyrosequencing in 837 Chinese Han patients with major trauma in Chongqing. We then validated the clinical relevance in 340 Zhejiang and 347 Yunnan patients. The expression of RAGE on the surface of peripheral blood mononuclear cells was measured by flow cytometric analysis. The results indicated that rs1800625 was significantly associated with sepsis morbidity rate and MODS in patients with major trauma in the Chongqing, Zhejiang and Yunnan districts. Patients with CC genotype had lower sepsis morbidity rate and MODS after major trauma. Furthermore, patients with CC genotype had significantly higher RAGE expression (P = 0.009). The rs1800625 polymorphism is a functional single nucleotide polymorphism and confers host susceptibility to sepsis and MODS in patients with major trauma.
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影响因子：
2
作者：
Wang, Zhengguo;Jiang, Jianxin
通讯作者：
Jiang, Jianxin
DOI：
10.1073/pnas.94.10.5296
发表时间：
1997-05-13
影响因子：
11.1
作者：
Yan, SD;Zhu, HJ;Schmidt, AM
通讯作者：
Schmidt, AM
影响因子：
2.9
作者：
Xue J;Ray R;Singer D;Böhme D;Burz DS;Rai V;Hoffmann R;Shekhtman A
通讯作者：
Shekhtman A
DOI：
10.1016/s0197-2456(98)00037-3
发表时间：
1998-12-01
期刊：
CONTROLLED CLINICAL TRIALS
影响因子：
--
作者：
Dupont, WD;Plummer, WD
通讯作者：
Plummer, WD
影响因子：
8.8
作者：
Levy, MM;Fink, MP;Ramsay, G
通讯作者：
Ramsay, G