Response of Methanogens in Arctic Sediments to Temperature and Methanogenic Substrate Availability.
Response of Methanogens in Arctic Sediments to Temperature and Methanogenic Substrate Availability.
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DOI：
10.1371/journal.pone.0129733
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Gray ND
中科院分区：
文献类型：
--
作者：
Blake LI;Tveit A;Øvreås L;Head IM;Gray ND
虽然寒冷环境是全球生物地球化学循环的主要贡献者，但对其微生物群落功能，结构和活动限制的了解相对较少。在这项研究中，一个基于微宇宙的方法被用来调查温度的影响，和产甲烷底物的修正案，（乙酸，甲醇和H2/CO2）对产甲烷菌的活性和产甲烷菌群落结构在高北极湿地（Solvatnet和Stuffallet，斯瓦尔巴群岛）。在Stuffallet沉积物微生态系统中没有检测到甲烷的产生（超过150天），而在Solvatnet沉积物微生态系统中（24小时内），温度从5 ° C到40 ° C，最高温度远远高于原位最高温度（夏季气温从-1.4 °C到14.1 ° C不等）。在不同的短期培养条件下，在Solvatnet产甲烷菌群落中观察到不同的反应。具体而言，在较高和较低温度下选择不同的群落。在较低温度（5 ° C）下，添加外源底物（乙酸盐、甲醇或H2/CO2）对产甲烷速率或产甲烷菌群落结构没有刺激作用。在这些孵育的社区占主导地位的甲烷调节科/WCHA 2 - 08家族水平组的成员，这是最相似的耐冷氢营养产甲烷菌沼泽甲烷球腔菌菌株E1 - 9c。相比之下，在较高的温度下，底物的修订提高甲烷生产在H2/CO2修订的微观世界，并发挥了明显的作用，在结构化产甲烷菌群落。具体而言，在30 ° C下，在与H2/CO2孵育后，甲烷调节科/WCHA 2 - 08的成员占优势，并且甲烷菌科和甲烷菌科响应于乙酸盐的添加而富集。这些结果可能表明，在短暂的寒冷环境中，产甲烷菌群落可以迅速响应温和的短期温度升高，但不一定是以前冻结的有机碳从解冻的冻土季节性释放。然而，随着温度的升高，这种碳的输入可能会对甲烷产生和产甲烷菌群落结构产生更大的影响。了解寒冷环境中厌氧微生物的作用和局限性，可以提供信息，可用于确定微生物过程中的区域特异性差异，最终控制甲烷通量到大气中。
Although cold environments are major contributors to global biogeochemical cycles, comparatively little is known about their microbial community function, structure, and limits of activity. In this study a microcosm based approach was used to investigate the effects of temperature, and methanogenic substrate amendment, (acetate, methanol and H2/CO2) on methanogen activity and methanogen community structure in high Arctic wetlands (Solvatnet and Stuphallet, Svalbard). Methane production was not detected in Stuphallet sediment microcosms (over a 150 day period) and occurred within Solvatnet sediments microcosms (within 24 hours) at temperatures from 5 to 40°C, the maximum temperature being at far higher than in situ maximum temperatures (which range from air temperatures of -1.4 to 14.1°C during summer months). Distinct responses were observed in the Solvatnet methanogen community under different short term incubation conditions. Specifically, different communities were selected at higher and lower temperatures. At lower temperatures (5°C) addition of exogenous substrates (acetate, methanol or H2/CO2) had no stimulatory effect on the rate of methanogenesis or on methanogen community structure. The community in these incubations was dominated by members of the Methanoregulaceae/WCHA2-08 family-level group, which were most similar to the psychrotolerant hydrogenotrophic methanogen Methanosphaerula palustris strain E1-9c. In contrast, at higher temperatures, substrate amendment enhanced methane production in H2/CO2 amended microcosms, and played a clear role in structuring methanogen communities. Specifically, at 30°C members of the Methanoregulaceae/WCHA2-08 predominated following incubation with H2/CO2, and Methanosarcinaceaeand Methanosaetaceae were enriched in response to acetate addition. These results may indicate that in transiently cold environments, methanogen communities can rapidly respond to moderate short term increases in temperature, but not necessarily to the seasonal release of previously frozen organic carbon from thawing permafrost soils. However, as temperatures increase such inputs of carbon will likely have a greater influence on methane production and methanogen community structure. Understanding the action and limitations of anaerobic microorganisms within cold environments may provide information which can be used in defining region-specific differences in the microbial processes; which ultimately control methane flux to the atmosphere.
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影响因子：
5.1
作者：
Casamayor, EO;Massana, R;Pedrós-Alió, C
通讯作者：
Pedrós-Alió, C
影响因子：
4.4
作者：
Ashelford, Kevin E.;Chuzhanova, Nadia A.;Weightman, Andrew J.
通讯作者：
Weightman, Andrew J.
影响因子：
4.4
作者：
Cadillo-Quiroz, Hinsby;Yashiro, Erica;Zinder, Stephen H.
通讯作者：
Zinder, Stephen H.
影响因子：
11
作者：
Hoj, Lone;Olsen, Rolf A.;Torsvik, Vigdis L.
通讯作者：
Torsvik, Vigdis L.
DOI：
10.1073/pnas.91.5.1609
发表时间：
1994-03-01
影响因子：
11.1
作者：
BARNS, SM;FUNDYGA, RE;PACE, NR
通讯作者：
PACE, NR