Dual-Functional Lipid Coating for the Nanopillar-Based Capture of Circulating Tumor Cells with High Purity and Efficiency.
Dual-Functional Lipid Coating for the Nanopillar-Based Capture of Circulating Tumor Cells with High Purity and Efficiency.
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DOI：
10.1021/acs.langmuir.6b03903
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发表时间：
2017-01-31
期刊：
影响因子：
--
通讯作者：
Cui B
中科院分区：
文献类型：
--
作者：
Lou HY;Zhao W;Hanson L;Zeng C;Cui Y;Cui B
循环肿瘤细胞（CTC）的临床研究对高捕获纯度和高捕获效率有着严格的要求。纳米结构表面已被证明可以显着提高捕获效率，但捕获纯度较低。在这里，我们介绍了双功能的脂质涂层的纳米结构表面。脂质涂层既作为有效的钝化层，有助于防止非特异性细胞粘附，又作为功能化层，用于基于抗体的特异性细胞捕获。此外，脂质双层的流动性使得抗体能够聚集，这增强了细胞-表面相互作用，以实现有效的细胞捕获。因此，脂质包被方法有助于提高基于纳米结构的CTC捕获的捕获效率和捕获纯度。
Clinical studies of circulating tumor cells (CTC) have stringent demands for high capture purity and high capture efficiency. Nanostructured surfaces have been shown to significantly increase the capture efficiency yet suffer from low capture purity. Here we introduce a dual-functional lipid coating on nanostructured surfaces. The lipid coating serves both as an effective passivation layer that helps prevent nonspecific cell adhesion and as a functionalized layer for antibody-based specific cell capture. In addition, the fluidity of lipid bilayers enables antibody clustering that enhances the cell–surface interaction for efficient cell capture. As a result, the lipid-coating method helps promote both the capture efficiency and capture purity of nanostructure-based CTC capture.
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影响因子：
14
作者：
Huang，Chun-Jen;Tseng，Po-Yuan;Chang，Ying-Chih
通讯作者：
Chang，Ying-Chih
DOI：
10.1073/pnas.0902621106
发表时间：
2009-08-04
影响因子：
11.1
作者：
Hartman, Nina C.;Nye, Jeffrey A.;Groves, Jay T.
通讯作者：
Groves, Jay T.
影响因子：
16.6
作者：
Lee, Hun Joo;Oh, Jin Ho;Huh, Nam
通讯作者：
Huh, Nam
影响因子：
--
作者：
Lin, Wan-Chen;Yu, Cheng-Han;Groves, Jay T
通讯作者：
Groves, Jay T
影响因子：
10.8
作者：
Dabkowska, Aleksandra P.;Niman, Cassandra S.;Nylander, Tommy
通讯作者：
Nylander, Tommy