Notch-responsive cells initiate the secondary transition in larval zebrafish pancreas.
Notch-responsive cells initiate the secondary transition in larval zebrafish pancreas.
复制标题
DOI：
10.1016/j.mod.2009.07.002
复制
发表时间：
2009-10
影响因子：
2.6
通讯作者：
Leach, Steven D.
中科院分区：
文献类型：
--
作者：
Parsons, Michael J.;Pisharath, Harshan;Yusuff, Shamila;Moore, John C.;Siekmann, Arndt F.;Lawson, Nathan;Leach, Steven D.
关键词：
斑马鱼为研究脊椎动物的发育提供了一个高度通用的模型。最近的许多研究已经阐明了斑马鱼胰腺器官发生的早期事件;然而，斑马鱼早期内分泌胰腺形成的几个方面与哺乳动物系统并不同源。为了更好地确定斑马鱼胰岛形成的机制，与哺乳动物中观察到的那些真正的同源性，我们在时间和空间上表征了斑马鱼次级胰岛形成。正如在小鼠中的情况一样，我们表明Notch抑制导致内分泌组织的早熟分化。此外，我们使用了转基因鱼表达荧光标记的Notch反应元件的控制下，观察这些诱导内分泌细胞的前体。这些胰腺Notch反应细胞代表了一种新的推定的祖细胞，与幼虫胰腺导管上皮细胞，这表明在斑马鱼的二次胰岛形成和哺乳动物的二次过渡之间的功能同源性。我们还表明，Notch反应细胞坚持在成人胰腺，并具有典型的特征，中央腺泡细胞，细胞类型被认为是一个多能祖细胞在成年哺乳动物胰腺。
Zebrafish provide a highly versatile model in which to study vertebrate development. Many recent studies have elucidated early events in the organogenesis of the zebrafish pancreas; however, several aspects of early endocrine pancreas formation in the zebrafish are not homologous to the mammalian system. To better identify mechanisms of islet formation in the zebrafish, with true homology to those observed in mammals, we have temporally and spatially characterized zebrafish secondary islet formation. As is the case in the mouse, we show that Notch inhibition leads to precocious differentiation of endocrine tissues. Furthermore, we have used transgenic fish expressing fluorescent markers under the control of a Notch-responsive element to observe the precursors of these induced endocrine cells. These pancreatic Notch-responsive cells represent a novel population of putative progenitors that are associated with larval pancreatic ductal epithelium, suggesting functional homology between secondary islet formation in zebrafish and the secondary transition in mammals. We also show that Notch-responsive cells persist in the adult pancreas and possess the classical characteristics of centroacinar cells, a cell type believed to be a multipotent progenitor cell in adult mammalian pancreas.
登录
查看更多内容
影响因子：
2.7
作者：
Davison, Jon M.;Akitake, Courtney M.;Parsons, Michael J.
通讯作者：
Parsons, Michael J.
影响因子：
64.8
作者：
Apelqvist, Å;Li, H;Edlund, H
通讯作者：
Edlund, H
影响因子：
4.6
作者：
Esni, F;Ghosh, B;Leach, SD
通讯作者：
Leach, SD
DOI：
10.1016/s0022-5320(62)80028-8
发表时间：
1962-01-01
期刊：
JOURNAL OF ULTRASTRUCTURE RESEARCH
影响因子：
--
作者：
EKHOLM, R;EDLUND, Y;ZELANDER, T
通讯作者：
ZELANDER, T
影响因子：
11.8
作者：
Chiang, MK;Melton, DA
通讯作者：
Melton, DA