Dynamic association with donor cell filopodia and lipid-modification are essential features of Wnt8a during patterning of the zebrafish neuroectoderm.
Dynamic association with donor cell filopodia and lipid-modification are essential features of Wnt8a during patterning of the zebrafish neuroectoderm.
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与供体细胞丝状伪足的动态关联和脂质修饰是斑马鱼神经外胚层模式化过程中 Wnt8a 的基本特征。
DOI：
10.1371/journal.pone.0084922
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Brand M
中科院分区：
文献类型：
--
作者：
Luz M;Spannl-Müller S;Özhan G;Kagermeier-Schenk B;Rhinn M;Weidinger G;Brand M
Wnt蛋白是一种保守的信号分子，在动物发育过程中调节模式的形成。许多Wnt蛋白通过添加脂质加合物进行后修饰。Wnt8a为脊椎动物发育中的神经板的前后轴模式化提供了关键信号。然而，目前尚不清楚这种蛋白质如何从其来源（胚盘边缘）传播到预期神经板中的靶细胞，以及脂质修饰如何影响Wnt8a的传播和活性。我们在活的斑马鱼胚胎中动态成像了生物活性的荧光标记Wnt8a。我们发现Wnt8a定位于活组织中的膜相关点状结构。在表达Wnt8a的细胞中，这些斑点位于丝状伪足细胞突起上，蛋白质可以从丝状伪足细胞突起释放。此外，发现Wnt8a与信号接收细胞上含有卷曲受体的簇共定位。结合体外和体内试验，我们比较了保守的Wnt8a残基在细胞和非细胞自主信号传导活性和分泌中的作用。非信号Wnt8变体显示这些残基可以调节Wnt8a在产生细胞膜和丝状伪足以及在接收组织中的分布。总之，我们的研究结果表明，Wnt8a形成动态集群上发现丝状伪足供体细胞和信号接收细胞膜。此外，它们证明了Wnt8a蛋白中的保守残基在神经外胚层图案化过程中在生产细胞和接收组织中的分布和信号传导活性的差异要求。
Wnt proteins are conserved signaling molecules that regulate pattern formation during animal development. Many Wnt proteins are post-translationally modified by addition of lipid adducts. Wnt8a provides a crucial signal for patterning the anteroposterior axis of the developing neural plate in vertebrates. However, it is not clear how this protein propagates from its source, the blastoderm margin, to the target cells in the prospective neural plate, and how lipid-modifications might influence Wnt8a propagation and activity. We have dynamically imaged biologically active, fluorescently tagged Wnt8a in living zebrafish embryos. We find that Wnt8a localizes to membrane-associated, punctate structures in live tissue. In Wnt8a expressing cells, these puncta are found on filopodial cellular processes, from where the protein can be released. In addition, Wnt8a is found colocalized with Frizzled receptor-containing clusters on signal receiving cells. Combining in vitro and in vivo assays, we compare the roles of conserved Wnt8a residues in cell and non-cell-autonomous signaling activity and secretion. Non-signaling Wnt8 variants show these residues can regulate Wnt8a distribution in producing cell membranes and filopodia as well as in the receiving tissue. Together, our results show that Wnt8a forms dynamic clusters found on filopodial donor cell and on signal receiving cell membranes. Moreover, they demonstrate a differential requirement of conserved residues in Wnt8a protein for distribution in producing cells and receiving tissue and signaling activity during neuroectoderm patterning.
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影响因子：
64.5
作者：
COUSO, JP;ARIAS, AM
通讯作者：
ARIAS, AM
影响因子：
4.1
作者：
Kurayoshi, Manabu;Yamamoto, Hideki;Kikuchi, Akira
通讯作者：
Kikuchi, Akira
MARCKS 在原肠胚形成运动期间皮质肌动蛋白动力学中的重要作用。
DOI：
10.1083/jcb.200310027
发表时间：
2004-01-19
期刊：
The Journal of cell biology
影响因子：
--
作者：
Iioka H;Ueno N;Kinoshita N
通讯作者：
Kinoshita N
影响因子：
2.5
作者：
KIMMEL, CB;BALLARD, WW;SCHILLING, TF
通讯作者：
SCHILLING, TF
影响因子：
3.7
作者：
Galli, Lisa M.;Burrus, Laura W.
通讯作者：
Burrus, Laura W.