Functional analysis of PrkA - a putative serine protein kinase from Mesorhizobium alhagi CCNWXJ12-2 - in stress resistance.
Functional analysis of PrkA - a putative serine protein kinase from Mesorhizobium alhagi CCNWXJ12-2 - in stress resistance.
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PrkA（来自中索根瘤菌 CCNWXJ12-2 的一种推定丝氨酸蛋白激酶）在抗逆性中的功能分析
DOI：
10.1186/s12866-016-0849-6
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发表时间：
2016-09-29
期刊：
影响因子：
4.2
通讯作者：
Wei G
中科院分区：
文献类型：
--
作者：
Liu X;Luo Y;Li Z;Wei G
丝/苏蛋白激酶是高度保守的激酶，在微生物中分布广泛，具有多种功能。骆驼刺中慢生根瘤菌CCNWXJ 12 -2是一株能与骆驼刺共生的α-变形菌，含有PrkA家族丝氨酸蛋白激酶PrkA。在我们以前的研究中，发现prkA的表达在高盐条件下下调。阐明M.构建了prkA缺失突变体alhagiPrkA，并对其表型进行了分析。测定了野生型菌株和prkA缺失突变株的耐盐碱性和抗氧化能力。结果表明，该缺失突变株比野生型菌株具有更高的耐盐性和耐碱性。测定了细胞总Na+含量，野生型菌株和突变体之间没有显着差异。prkA缺失突变体也表现出比野生型菌株更高的H2 O2耐受性。因此，抗氧化酶的活性进行了测定。过氧化氢酶活性在野生型和缺失突变体中相似，而超氧化物歧化酶活性在突变体中高于野生型。我们首先分析了丝氨酸蛋白激酶PrkA在M.阿尔哈吉。我们的数据表明PrkA可以降低M. alhagi在环境胁迫和prkA基因缺失的情况下，显著提高了M.阿尔哈吉。
Serine/threonine protein kinases are highly conserved kinases with a wide distribution in microbes and with multiple functions. Mesorhizobium alhagi CCNWXJ12-2, a α-proteobacterium which could be able to form symbiosis with Alhagi sparsifolia in northwest of China, contains a putative PrkA-family serine protein kinase, PrkA. In our previous study, the expression of prkA was found to be downregulated in high-salt conditions. To elucidate the function of M. alhagi PrkA, a prkA deletion mutant was constructed and the phenotypes of the mutant were analyzed. The salt and alkaline tolerance and antioxidant capacity of the wild-type strain and the prkA deletion mutant was measured. Our results showed that the deletion mutant had higher salt and alkaline tolerance than the wild-type strain. The total cellular Na+ content was measured and showed no significant difference between the wild-type strain and the mutant. The prkA deletion mutant also showed a higher H2O2 tolerance than the wild-type strain. Therefore the activities of antioxidant enzymes were measured. Catalase activity was similar in the wild-type and the deletion mutant, while the superoxide dismutase activity in the mutant was higher than that in the wild-type. We firstly analyze the function of a serine protein kinase, PrkA, in M. alhagi. Our data indicate that PrkA could reduce the survival of M. alhagi under environmental stress and deletion of prkA dramatically improved the salt and alkaline tolerance and antioxidant capacity of M. alhagi.
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影响因子：
5.7
作者：
Harrison A;Bakaletz LO;Munson RS Jr
通讯作者：
Munson RS Jr
DOI：
10.1099/ijs.0.014043-0
发表时间：
2010-04-01
影响因子：
2.8
作者：
Chen, Wei-Min;Zhu, Wen-Fei;Wei, Ge Hong
通讯作者：
Wei, Ge Hong
影响因子：
3
作者：
Koh, Myung Jin;Lee, Hyun Sung;Choi, Sang Ho
通讯作者：
Choi, Sang Ho
影响因子：
12.3
作者：
Vercruysse M;Fauvart M;Jans A;Beullens S;Braeken K;Cloots L;Engelen K;Marchal K;Michiels J
通讯作者：
Michiels J
影响因子：
2.8
作者：
Kumar, Dinesh;Palaniyandi, Kannan;Narayanan, Sujatha
通讯作者：
Narayanan, Sujatha