Tenogenesis of Decellularized Porcine Achilles Tendon Matrix Reseeded with Human Tenocytes in the Nude Mice Xenograft Model.
Tenogenesis of Decellularized Porcine Achilles Tendon Matrix Reseeded with Human Tenocytes in the Nude Mice Xenograft Model.
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DOI：
10.3390/ijms19072059
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发表时间：
2018-07-15
影响因子：
5.6
通讯作者：
Schulze-Tanzil G
中科院分区：
文献类型：
--
作者：
Lohan A;Kohl B;Meier C;Schulze-Tanzil G
在无细胞异种细胞外肌腱基质（xECM）中培养自体人肌腱细胞可能是肌腱重建的一种方法。本研究的目的是通过将脱细胞猪跟腱与人腘绳肌腱来源的腱细胞再细胞化植入裸鼠体内来实现肌腱样组织的形成。脱细胞xECM的结构进行了组织学监测，然后与人肌腱细胞动态restellate。在体内6-12周后，使用宏观和组织学评分系统、活力测定和定量DNA和糖胺聚糖（GAG）测定来监测构建体质量。为了与肌腱xECM进行比较，以类似的方式植入合成聚乙醇酸（PGA）聚合物。尽管脱细胞的xECM失去了一些GAG和结构，但它可以在体外与人腱细胞再细胞化，但细胞分布仍然不均匀，在构建体的边缘积累。在体内，与PGA相比，xECM构建体显示尺寸没有改变，没有炎症和包囊，并且细胞分布更均匀。与无细胞植入构建体相比，具有腱细胞的xECM重建显示出上级组织学质量，并且包含存活的人细胞。在体内6周和12周后，它们的DNA含量类似于体外再细胞化的天然肌腱和xECM。结果表明，再生脱细胞xECM在体内形成肌腱样组织。
Cultivation of autologous human tenocytes in a cell-free xenogenic extracellular tendon matrix (xECM) could present an approach for tendon reconstruction. The aim of this study was to achieve tendon-like tissue formation by implanting decellularized porcine Achilles tendons recellularized with human hamstring tendon-derived tenocytes into nude mice. The structure of decellularized xECM was histologically monitored before being dynamically reseeded with human tenocytes. After 6–12 weeks in vivo, construct quality was monitored using macroscopical and histological scoring systems, vitality assay and quantitative DNA and glycosaminoglycan (GAG) assays. For comparison to tendon xECM, a synthetic polyglycolic acid (PGA) polymer was implanted in a similar manner. Despite decellularized xECM lost some GAGs and structure, it could be recellularized in vitro with human tenocytes, but the cell distribution remained inhomogeneous, with accumulations at the margins of the constructs. In vivo, the xECM constructs revealed in contrast to the PGA no altered size, no inflammation and encapsulation and a more homogeneous cell distribution. xECM reseeded with tenocytes showed superior histological quality than cell-free implanted constructs and contained surviving human cells. Their DNA content after six and 12 weeks in vivo resembled that of native tendon and xECM recellularized in vitro. Results suggest that reseeded decellularized xECM formed a tendon-like tissue in vivo.
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DOI：
10.1002/jbm.b.33786
发表时间：
2017-11
期刊：
Journal of biomedical materials research. Part B, Applied biomaterials
影响因子：
--
作者：
Edwards JH;Herbert A;Jones GL;Manfield IW;Fisher J;Ingham E
通讯作者：
Ingham E
影响因子：
2.3
作者：
Lohan, A.;Marzahn, U.;John, T.
通讯作者：
John, T.
影响因子：
2.9
作者：
Lohan, Anke;Stoll, Christiane;Schulze-Tanzil, Gundula
通讯作者：
Schulze-Tanzil, Gundula
影响因子：
3.6
作者：
McGoldrick, Rory;Chattopadhyay, Arhana;Chang, James
通讯作者：
Chang, James
影响因子：
--
作者：
Ingram, Joanne Helen;Korossis, Sotirios;Ingham, Eileen
通讯作者：
Ingham, Eileen