The effects of irradiation on the biological and biomechanical properties of an acellular porcine superflexor tendon graft for cruciate ligament repair.
The effects of irradiation on the biological and biomechanical properties of an acellular porcine superflexor tendon graft for cruciate ligament repair.
复制标题
DOI：
10.1002/jbm.b.33786
复制
发表时间：
2017-11
期刊：
影响因子：
--
通讯作者：
Ingham E
中科院分区：
文献类型：
--
作者：
Edwards JH;Herbert A;Jones GL;Manfield IW;Fisher J;Ingham E
脱细胞异种组织有可能为前交叉韧带（ACL）修复提供“现成”移植物。为了确保这种移植物在包装后是无菌的，期望使用最终灭菌方法。在这里，γ射线和电子束照射对先前开发的脱细胞猪超屈肌腱（pSFT）的生物学和生物力学性能的影响进行了研究。将过氧乙酸处理后的辐照与单独过氧乙酸处理进行比较，并评估移植物在长期储存后的稳定性。辐照不影响移植物的总胶原含量或生物相容性（使用接触细胞毒性试验测定），但略微增加了变性胶原的量，并以剂量依赖性方式降低了组织的热变性温度。移植物的生物力学特性通过辐照而改变（极限拉伸强度和杨氏模量降低，失效应变增加），但仍上级报告的天然人ACL的特性。在4°C下长期储存对移植物没有负面影响。在所有测试条件中，最小25 kGy剂量的伽马辐照对移植物的影响最小，表明该剂量产生具有足够ACL修复机械性能的生物相容性pSFT移植物。© 2016 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater，105 B：2477-2486，2017。
Acellular xenogeneic tissues have the potential to provide ‘off‐the‐shelf’ grafts for anterior cruciate ligament (ACL) repair. To ensure that such grafts are sterile following packaging, it is desirable to use terminal sterilization methods. Here, the effects of gamma and electron beam irradiation on the biological and biomechanical properties of a previously developed acellular porcine superflexor tendon (pSFT) were investigated. Irradiation following treatment with peracetic acid was compared to peracetic acid treatment alone and the stability of grafts following long‐term storage assessed. Irradiation did not affect total collagen content or biocompatibility (determined using a contact cytotoxicity assay) of the grafts, but slightly increased the amount of denatured collagen in and decreased the thermal denaturation temperature of the tissue in a dose dependant fashion. Biomechanical properties of the grafts were altered by irradiation (reduced ultimate tensile strength and Young's modulus, increased failure strain), but remained superior to reported properties of the native human ACL. Long term storage at 4°C had no negative effects on the grafts. Of all the conditions tested, a dose of minimum 25 kGy of gamma irradiation had least effect on the grafts, suggesting that this dose produces a biocompatible pSFT graft with adequate mechanical properties for ACL repair. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2477–2486, 2017.
登录
查看更多内容
影响因子：
6.1
作者：
Frankel, EN;Huang, SW;Prior, E
通讯作者：
Prior, E
影响因子：
4.6
作者：
Ketchedjian, A;Jones, AL;Hopkins, R
通讯作者：
Hopkins, R
影响因子：
1.5
作者：
Lomas, R. J.;Jennings, L. M.;Kearney, J. N.
通讯作者：
Kearney, J. N.
影响因子：
3.7
作者：
Greaves NS;Lqbal SA;Morris J;Benatar B;Alonso-Rasgado T;Baguneid M;Bayat A
通讯作者：
Bayat A
影响因子：
3.8
作者：
Londono, Ricardo;Badylak, Stephen F.
通讯作者：
Badylak, Stephen F.