Visualisation and characterisation of mononuclear phagocytes in the chicken respiratory tract using CSF1R-transgenic chickens.
Visualisation and characterisation of mononuclear phagocytes in the chicken respiratory tract using CSF1R-transgenic chickens.
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DOI：
10.1186/s13567-018-0598-7
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发表时间：
2018-10-10
影响因子：
4.4
通讯作者：
Vervelde L
中科院分区：
文献类型：
--
作者：
Sutton K;Costa T;Alber A;Bryson K;Borowska D;Balic A;Kaiser P;Stevens M;Vervelde L
呼吸道是许多禽类病原体的关键器官，也是家禽业中疫苗接种的主要途径。为了通过增加疫苗的摄取和靶向抗原呈递细胞来改善鸡接种疫苗后的免疫应答，需要更好地了解禽类呼吸道免疫系统。使用在单核吞噬细胞（MNP）谱系的细胞中在CSF 1 R启动子和增强子的控制下表达报告基因（eGFP或mApple）的转基因MacReporter鸟，以观察从胚胎第18-63周龄的鸟的气管、肺和气囊中淋巴组织、巨噬细胞和树突细胞的个体发育。CSF 1 R-转基因+细胞的小聚集体在1周龄时开始在次级支气管的开口处形成，表明有组织的支气管相关淋巴组织的早期发育。免疫组织化学染色显示MNP在肺中的亚群，基于CSF 1 R转基因、CD 11、TIM 4、LAMP 1和MHC II的表达。覆盖支气管相关淋巴组织的特化上皮细胞或M细胞表达CSF 1 R转基因，II型肺细胞表达LAMP 1，表明这些上皮细胞是吞噬和转胞吞抗原。从4周开始在气管中观察到高度组织化的淋巴组织。在所有年龄段的整个气囊中，CSF 1 R-转基因+细胞分散，在后期阶段，CSF 1 R-转基因+细胞内衬毛细血管。这些结果将作为进一步的功能表征的巨噬细胞和树突状细胞及其在呼吸系统疾病和疫苗反应中的作用的基础。本文的在线版本（10.1186/s13567-018-0598-7）包含补充材料，可供授权用户使用。
The respiratory tract is a key organ for many avian pathogens as well as a major route for vaccination in the poultry industry. To improve immune responses after vaccination of chickens through increased uptake of vaccines and targeting to antigen presenting cells, a better understanding of the avian respiratory immune system is required. Transgenic MacReporter birds were used expressing a reporter gene (eGFP or mApple) under the control of the CSF1R promoter and enhancer in cells of the mononuclear phagocyte (MNP) lineage to visualize the ontogeny of the lymphoid tissue, macrophages and dendritic cells, in the trachea, lung and air sac of birds from embryonic day 18–63 weeks of age. Small aggregates of CSF1R-transgene+ cells start to form at the openings of the secondary bronchi at 1 week of age, indicative of the early development of the organised bronchus-associated lymphoid tissue. Immunohistochemical staining revealed subpopulations of MNPs in the lung, based on expression of CSF1R-transgene, CD11, TIM4, LAMP1, and MHC II. Specialised epithelial cells or M cells covering the bronchus-associated lymphoid tissue expressed CSF1R-transgene and type II pneumocytes expressed LAMP1 suggesting that these epithelial cells are phagocytic and transcytose antigen. Highly organised lymphoid tissue was seen in trachea from 4 weeks onwards. Throughout the air sacs at all ages, CSF1R-transgene+ cells were scattered and at later stages, CSF1R-transgene+ cells lined capillaries. These results will serve as a base for further functional characterization of macrophages and dendritic cells and their role in respiratory diseases and vaccine responses. The online version of this article (10.1186/s13567-018-0598-7) contains supplementary material, which is available to authorized users.
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影响因子：
7.3
作者：
Baharom F;Rankin G;Blomberg A;Smed-Sörensen A
通讯作者：
Smed-Sörensen A
影响因子：
1.8
作者：
Dwars, R. Marius;Matthijs, Mieke G. R.;Landman, Wil J. M.
通讯作者：
Landman, Wil J. M.
影响因子：
2.9
作者：
Cuperus, Tryntsje;van Dijk, Albert;Haagsman, Henk P.
通讯作者：
Haagsman, Henk P.
影响因子：
3.3
作者：
Hogenkamp, Astrid;Isohadouten, Najiha;Haagsman, Henk P.
通讯作者：
Haagsman, Henk P.
影响因子：
3.6
作者：
JEURISSEN, SHM;JANSE, EM;DEBOER, GF
通讯作者：
DEBOER, GF