Time-dependent effects of isoflurane and dexmedetomidine on functional connectivity, spectral characteristics, and spatial distribution of spontaneous BOLD fluctuations.
Time-dependent effects of isoflurane and dexmedetomidine on functional connectivity, spectral characteristics, and spatial distribution of spontaneous BOLD fluctuations.
复制标题
DOI：
10.1002/nbm.3062
复制
发表时间：
2014-03
影响因子：
2.9
通讯作者：
Keilholz, Shella Dawn
中科院分区：
文献类型：
--
作者：
Magnuson, Matthew Evan;Thompson, Garth John;Pan, Wen-Ju;Keilholz, Shella Dawn
关键词：
在啮齿动物模型中进行fMRI实验通常需要麻醉;然而，常用的麻醉方案可能会在研究期间表现出大脑状况的变化。目前的工作探讨了这种可能性。11只大鼠用2%异氟烷麻醉; 4只大鼠短期麻醉（30 min，模拟诱导和fMRI设置），7只大鼠长期麻醉（3 h，模拟手术准备）。初始麻醉期后，停用异氟烷，并给予右美托咪定推注（0.025 mg/kg）和连续皮下输注（0.05 mg/kg/h）。从右美托咪定推注后0.75 h至推注后5.75 h，每30 min进行一次血氧水平依赖性静息状态成像。从初级体感皮层的时间过程中获得的功率谱的评价显示，在一般情况下，在两组的静息状态成像的持续时间内，低频功率（0.05-0.3 Hz）单调增加。在前2.75 h，短异氟烷组中存在较大的低频带光谱功率（0.05-0.15 Hz），但在3.25 h时光谱变得高度均匀。在3.75 h时间点开始出现约0.18 Hz峰值，两组均存在，并且演变相似，强度随着右美托咪定镇静持续时间（以及自异氟烷停药后的时间）延长而增加。仅在长时间异氟烷组中，双侧功能连接随着麻醉持续时间的增加而增强，相关性与低波段光谱功率呈线性关系。短异氟烷组和长异氟烷组之间的连接性和光谱指标在约3.25 h时收敛，表明异氟烷的影响已经消退。在异氟烷后使用右美托咪定进行功能研究的研究者应了解扫描前异氟烷持续时间的持续时间特异性影响以及右美托咪定长期成像的持续影响。
Anesthesia is often necessary to perform fMRI experiments in the rodent model; however, commonly used anesthetic protocols may manifest changing brain conditions over the duration of the study. This possibility was explored in the current work. Eleven rats were anesthetized with 2% isoflurane anesthesia; four rats were anesthetized for a short period (30min, simulating induction and fMRI setup) and seven rats were anesthetized for a long period (3 h, simulating surgical preparation). Following the initial anesthetic period, isoflurane was discontinued, and a dexmedetomidine bolus (0.025 mg/kg) and continuous subcutaneous infusion (0.05 mg/kg/h) were administered. Blood-oxygen-level dependent resting state imaging was performed every 30 min from 0.75 h post dexmedetomidine bolus until 5.75 h post-bolus. Evaluation of power spectra obtained from time courses in the primary somatosensory cortex revealed, in general, a monotonic increase in low-frequency power (0.05–0.3 Hz) in both groups over the duration of resting state imaging. Greater low-band spectral power (0.05–0.15 Hz) is present in the short isoflurane group for the first 2.75 h, but the spectra become highly uniform at 3.25 h. The emergence of a ~0.18 Hz peak, beginning at the 3.75 h time point, exists in both groups and evolves similarly, increasing in strength as the duration of dexmedetomidine sedation (and time since isoflurane cessation) extends. In the long isoflurane group only, bilateral functional connectivity strengthens with anesthetic duration, and correlation is linearly linked to low-band spectral power. Convergence of connectivity and spectral metrics between the short and long isoflurane groups occurs at ~3.25 h, suggesting the effects of isoflurane have subsided. Researchers using dexmedetomidine following isoflurane for functional studies should be aware of the duration specific effects of the pre-scan isoflurane durations as well as the continuing influences of long-term imaging under dexmedetomidine.
登录
查看更多内容
影响因子：
1.7
作者：
Antunes, LM;Golledge, HD;Flecknell, PA
通讯作者：
Flecknell, PA
DOI：
10.1152/ajpheart.1997.272.6.h2547
发表时间：
1997-06-01
影响因子：
4.8
作者：
Asano, Y;Koehler, RC;McPherson, RW
通讯作者：
McPherson, RW
影响因子：
4.4
作者：
Magnuson, Matthew;Majeed, Wacjas;Keilholz, Sheila D.
通讯作者：
Keilholz, Sheila D.
影响因子：
2.2
作者：
Granholm, M.;McKusick, B. C.;Aspegren, J. C.
通讯作者：
Aspegren, J. C.
影响因子：
3
作者：
Guilfoyle, David N.;Gerum, Scott V.;Sanchez, Jamie L.;Balla, Andrea;Sershen, Henry;Javitt, Daniel C.;Hoptman, Matthew J.
通讯作者：
Hoptman, Matthew J.