Salivary DNA Loads for Human Herpesviruses 6 and 7 Are Correlated With Disease Phenotype in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome.
Salivary DNA Loads for Human Herpesviruses 6 and 7 Are Correlated With Disease Phenotype in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome.
复制标题
DOI：
10.3389/fmed.2021.656692
复制
发表时间：
2021
影响因子：
3.9
通讯作者：
Cliff JM
中科院分区：
文献类型：
--
作者：
Lee JS;Lacerda EM;Nacul L;Kingdon CC;Norris J;O'Boyle S;Roberts CH;Palla L;Riley EM;Cliff JM
肌痛性脑脊髓炎/慢性疲劳综合征(ME/CFS)是一种复杂的慢性疾病，累及多个身体系统，病因不明，发病机制不明，症状起伏不定，可导致严重的虚弱。它经常被报道是由感染引发的，但在ME/CFS患者和健康人之间，接触任何特定病毒或血清阳性率没有明显差异。然而，由于疱疹病毒以潜伏的形式持续存在，并周期性地重新激活，因此一直被反复假设为MS/CFS慢性复发/缓解型的基础。ME/CFS可能与疱疹病毒的重新激活有关，以前由于检测方法不够灵敏而无法检测到这种情况。唾液样本来自30名患有ME/CFS的患者，每月间隔6个月，当他们症状恶化时，以及来自14名健康对照个体。用数字液滴聚合酶链式反应检测9株人类疱疹病毒的DNA载量。在每个时间点采用问卷调查的方式进行症状评定。在唾液标本中检出人类疱疹病毒6B、HHV-7、单纯疱疹病毒1型和EB病毒，ME/CFS患者的HHV-6B和HHV-7病毒载量高于健康对照组。患有ME/CFS的参与者可以大致分为两组：一组在6个月内表现出可检测到的疱疹病毒的波动模式，而第二组表现出更稳定的病毒表现。在第一组中，HHV-6B和HHV-7病毒载量与疼痛、神经认知和自主神经功能障碍等症状评分的严重程度呈正相关。结果表明，病毒DNA载量波动与ME/CFS症状相关：这与发病机制与疱疹病毒重新激活状态有关的假设是一致的，这一假设应该得到正式检验。疱疹病毒的重新激活可能是ME/CFS免疫功能紊乱的原因或结果。这里开发的抽样策略和分子工具允许进行如此大规模的流行病学调查。
Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a complex chronic condition affecting multiple body systems, with unknown cause, unclear pathogenesis mechanisms, and fluctuating symptoms which may lead to severe debilitation. It is frequently reported to have been triggered by an infection, but there are no clear differences in exposure to, or seroprevalence of, any particular viruses between people with ME/CFS and healthy individuals. However, herpes viruses have been repeatedly hypothesized to underlie the chronic relapsing/remitting form of MS/CFS due to their persistence in a latent form with periodic reactivation. It is possible that ME/CFS is associated with herpes virus reactivation, which has not been detectable previously due to insufficiently sensitive testing methods. Saliva samples were collected from 30 people living with ME/CFS at monthly intervals for 6 months and at times when they experienced symptom exacerbation, as well as from 14 healthy control individuals. The viral DNA load of the nine humanherpes viruses was determined by digital droplet PCR. Symptoms were assessed by questionnaire at each time point. Human herpesvirus (HHV) 6B, HHV-7, herpes simplex virus 1 and Epstein-Barr virus were detectable within the saliva samples, with higher HHV-6B and HHV-7 viral loads detected in people with ME/CFS than in healthy controls. Participants with ME/CFS could be broadly separated into two groups: one group displayed fluctuating patterns of herpesviruses detectable across the 6 months while the second group displayed more stable viral presentation. In the first group, there was positive correlation between HHV-6B and HHV-7 viral load and severity of symptom scores, including pain, neurocognition, and autonomic dysfunction. The results indicate that fluctuating viral DNA load correlates with ME/CFS symptoms: this is in accordance with the hypothesis that pathogenesis is related to herpesvirus reactivation state, and this should be formally tested. Herpesvirus reactivation might be a cause or consequence of dysregulated immune function seen in ME/CFS. The sampling strategy and molecular tools developed here permit such large-scale epidemiological investigations.
登录
查看更多内容
EB 病毒 (EBV) 相关肌痛性脑脊髓炎/慢性疲劳综合征 (ME/CFS) 中的早期生长反应基因上调。
影响因子：
5.5
作者：
Kerr J
通讯作者：
Kerr J
影响因子：
8.8
作者：
Ablashi, DV;Eastman, HB;Whitman, JE
通讯作者：
Whitman, JE
影响因子：
2.6
作者：
Chu, Lily;Valencia, Ian J.;Montoya, Jose G.
通讯作者：
Montoya, Jose G.
DOI：
10.1073/pnas.93.18.9788
发表时间：
1996-09-03
影响因子：
11.1
作者：
Katsafanas, GC;Schirmer, EC;Frenkel, N
通讯作者：
Frenkel, N
影响因子：
3.1
作者：
KOMAROFF, AL
通讯作者：
KOMAROFF, AL