Identical distribution of fluorescently labeled brain and muscle actins in living cardiac fibroblasts and myocytes.
Identical distribution of fluorescently labeled brain and muscle actins in living cardiac fibroblasts and myocytes.
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活着的心脏成纤维细胞和心肌细胞中荧光标记的脑和肌肉肌动蛋白的相同分布。
DOI：
10.1083/jcb.100.1.292
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发表时间：
1985-01
期刊：
影响因子：
--
通讯作者：
Wang YL
中科院分区：
文献类型：
--
作者：
McKenna N;Meigs JB;Wang YL
我们研究了活的肌肉和非肌肉细胞是否可以区分显微注射的肌肉和非肌肉肌动蛋白。将从兔背部和腿部肌肉中纯化并用异硫氰酸荧光素标记的肌肉肌动蛋白和从羔羊脑中纯化并用丽丝胺罗丹明B磺酰氯标记的非肌肉肌动蛋白共同注射到鸡胚心肌细胞和成纤维细胞中。当使用对异硫氰酸荧光素或丽丝胺罗丹明 B 磺酰氯具有选择性的滤光片组比较两种肌动蛋白的荧光图像时，在肌肉和非肌肉细胞中检测到基本上相同的分布模式。特别是，我们没有发现在这种分辨率水平下，任何结构显示出肌肉或非肌肉肌动蛋白的优先结合。在成纤维细胞中，两种肌动蛋白主要与应力纤维和褶边相关。在肌细胞中，两种肌动蛋白都位于肌节中。此外，通过荧光鬼笔环肽或鬼笔环肽染色显示，含有显微注射肌动蛋白的结构的分布与含有内源性F-肌动蛋白的结构的分布相似。我们的结果表明，至少在这些实验条件下，肌肉和非肌肉细胞中的肌动蛋白结合位点不会区分不同形式的肌动蛋白。
We have investigated whether living muscle and nonmuscle cells can discriminate between microinjected muscle and nonmuscle actins. Muscle actin purified from rabbit back and leg muscles and labeled with fluorescein isothiocyanate, and nonmuscle actin purified from lamb brain and labeled with lissamine rhodamine B sulfonyl chloride, were co- injected into chick embryonic cardiac myocytes and fibroblasts. When fluorescence images of the two actins were compared using filter sets selective for either fluorescein isothiocyanate or lissamine rhodamine B sulfonyl chloride, essentially identical patterns of distribution were detected in both muscle and nonmuscle cells. In particular, we found no structure that, at this level of resolution, shows preferential binding of muscle or nonmuscle actin. In fibroblasts, both actins are associated primarily with stress fibers and ruffles. In myocytes, both actins are localized in sarcomeres. In addition, the distribution of structures containing microinjected actins is similar to that of structure containing endogenous F-actin, as revealed by staining with fluorescent phalloidin or phallacidin. Our results suggest that, at least under these experimental conditions, actin- binding sites in muscle and nonmuscle cells do not discriminate among different forms of actins.
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影响因子：
7.8
作者：
MCKEITHAN, TW;ROSENBAUM, JL
通讯作者：
ROSENBAUM, JL
DOI：
10.1080/00327488108061774
发表时间：
1981-01-01
期刊：
PREPARATIVE BIOCHEMISTRY
影响因子：
--
作者：
RUSCHA, MF;HIMES, RH
通讯作者：
HIMES, RH
DOI：
10.1073/pnas.75.2.857
发表时间：
1978-01-01
影响因子：
11.1
作者：
TAYLOR, DL;WANG, YL
通讯作者：
WANG, YL
DOI：
10.1073/pnas.74.1.120
发表时间：
1977-01-01
影响因子：
11.1
作者：
RUBENSTEIN, PA;SPUDICH, JA
通讯作者：
SPUDICH, JA
影响因子：
64.5
作者：
GUNNING, P;PONTE, P;SKOULTCHI, AI
通讯作者：
SKOULTCHI, AI