Circular dichroism studies on single Chinese hamster cells.
Circular dichroism studies on single Chinese hamster cells.
复制标题
中国仓鼠单个细胞的圆二色性研究。
DOI：
10.1021/bi00340a600
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发表时间：
1985
期刊：
影响因子：
2.9
通讯作者：
Bustamante,C
中科院分区：
文献类型：
--
作者：
Maestre,MF;Salzman,GC;Tobey,RA;Bustamante,C
摘要：测定了中国仓鼠(CHO)单个细胞的圆二色谱(CD)和圆强度差分散射(CID)对圆二色谱(CD)的贡献随细胞周期中所处位置的变化。数据在三个主要光谱区进行分析：(1)290 nm以上的区域(散射区)，(2)250-290 nm的区域(核酸吸收区域)，(3)240 nm以下的区域(蛋白质吸收区域)。结果表明，CD/CIDS显微分光光度法是一种较好的细胞周期时相指示剂。结果与染色质是以手性超结构组织的观点一致，当细胞经过其周期时，这些超结构以差分方式散射圆偏振光。(圆二色谱(CD)是一种对生物大分子的一级、二级和三级结构敏感的光谱技术。它测量了样品在用右圆偏振光和左圆偏振光连续照射时吸收截面的差异。这项技术已被应用于研究各种结构，从核酸单体级别的小手性分子，到溶液中完整的细菌细胞、细胞核和真核细胞的结构(Tinoo等人，1980年)。在简单的手性组织的情况下，例如小的光学活性分子，对结果的解释相当直接。然而，由于散射贡献的存在，结果变得复杂起来
Chemistry Department, The University of New Mexico, Albuquerque, New Mexico 87177 Received December 18, 1984 abstract: The circular dichroism (CD) and circular intensity differential scattering (CIDS) contributions to the CD of Single Chinesehamster (CHO) cells have been measured as a function of the position in the cell cycle. The data are analyzed in three main spectral regions:(1) the region above 290 nm (scattering region),(2) the 250-290-nm regions (nucleic acid absorption region), and (3) the region below 240 nm (protein absorption region). The results show that CD/CIDS microspectrophotometry is a good indicator of the cell cycle phase. The results are consistent with the view that chromatin is organized in chiral superstructures which differentially scatter circularly polarized light, as the cell passes through its cycle.(Circular dichroism (CD) is a spectroscopic technique sen-sitive to primary, secondary, and tertiary structures of biological macromolecules. It measures the difference in ab-sorption cross section exhibited by a sample when it is illu-minated successively with right-and left-circularly polarized light. The technique has been applied to the study of structures ranging from small chiral molecules, of the order of nucleic acid monomers, to structures as large as intact bacterial cells, cell nuclei, and eukaryotic cells in solutions (Tinoco et al., 1980). Interpretation of results hadbeen reasonably straightforward in cases of simple chiral organizations, such as small optically active molecules. However, results havebeen complicated by thepresence of scattering contributions and
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20 分钟内完成细胞周期分析
影响因子：
56.9
作者：
H. Crissman;R. Tobey
通讯作者：
R. Tobey
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DOI：
--
发表时间：
1980
期刊：
影响因子：
--
作者：
C. Bustamante;M. Maestre;I. Tinoco
通讯作者：
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影响因子：
3.7
作者：
D. Holm;L. Cram
通讯作者：
L. Cram
影响因子：
4.4
作者：
BUSTAMANTE, C;TINOCO, I;MAESTRE, MF
通讯作者：
MAESTRE, MF
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影响因子：
2.9
作者：
D. Gray;T. N. Taylor;D. Lang
通讯作者：
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