MicroRNA-181a is involved in the regulation of human endometrial stromal cell decidualization by inhibiting Krüppel-like factor 12.
MicroRNA-181a is involved in the regulation of human endometrial stromal cell decidualization by inhibiting Krüppel-like factor 12.
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MicroRNA-181a通过抑制Kruppel样因子12参与调节人子宫内膜基质细胞蜕膜化
DOI：
10.1186/s12958-015-0019-y
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发表时间：
2015-03-26
期刊：
影响因子：
--
通讯作者：
Hu Y
中科院分区：
文献类型：
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作者：
Zhang Q;Zhang H;Jiang Y;Xue B;Diao Z;Ding L;Zhen X;Sun H;Yan G;Hu Y
子宫内膜向蜕膜的转化是囊胚正常着床的必要条件。然而，转录后调控和参与去个体化的mirna仍然知之甚少。在这里，我们检测了microRNA-181a （miR-181a）在去个体化人子宫内膜基质细胞（hESC）中的表达。此外，我们在体外研究了miR-181a对hESC去个体化的功能作用。采用实时荧光定量PCR （qRT-PCR）检测miR-181a在去个体化hESC中的表达。通过qRT-PCR、酶联荧光法和免疫荧光法研究miR-181a在hESC中表达增强或抑制后去个性化标记基因的表达。采用荧光素酶报告基因法、western blotting、qRT-PCR和免疫荧光法鉴定miR-181a与kr<s:1> ppel样因子12 （KLF12）之间的关系。miR-181a表达水平在8-Br-cAMP和MPA处理的hESC中显著升高。miR-181a表达增加促进hESC去个体化相关基因表达和形态转化；相反，抑制miR-181a表达会在体外损害hESC去个体化。进一步分析证实，miR-181a与转录因子KLF12的3 '非翻译区相互作用，并在转录和翻译水平下调KLF12。KLF12过表达可消除mir -181a诱导的去个体化。我们的研究结果表明，miR-181a通过抑制KLF12在体外人子宫内膜间质细胞脱胞过程中发挥重要的功能作用。
The transformation of endometrium into decidua is essential for normal implantation of the blastocyst. However, the post-transcriptional regulation and the miRNAs involved in decidualization remain poorly understood. Here, we examined microRNA-181a (miR-181a) expression in decidualized human endometrial stromal cell (hESC). In addition, we investigated the functional effect of miR-181a on hESC decidualization in vitro. Quantitative real-time PCR (qRT-PCR) was used to detect the profile of miR-181a in decidualized hESC. qRT-PCR, enzyme-linked fluorescent assay, and immunofluorescence assay were performed to investigate decidualization marker genes’ expression after enhancing or inhibition of miR-181a expression in hESC. Luciferase reporter assay, western blotting, qRT-PCR, and immunofluorescence assay were carried out to identify the relationship between miR-181a and Krüppel-like factor 12 (KLF12). miR-181a expression levels increased dramatically in hESC treated with 8-Br-cAMP and MPA. Increased miR-181a expression promoted hESC decidualization-related gene expression and morphological transformation; conversely, inhibition of miR-181a expression compromised hESC decidualization in vitro. Further analysis confirmed that miR-181a interacted with the 3′ untranslated region of the transcription factor KLF12 and down-regulated KLF12 at the transcriptional and translational levels. KLF12 overexpression abolished miR-181a-induced decidualization. Our findings suggest that miR-181a plays a functionally important role in human endometrial stromal cell decidualization in vitro by inhibiting KLF12.
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影响因子：
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作者：
Konno Y;Dong P;Xiong Y;Suzuki F;Lu J;Cai M;Watari H;Mitamura T;Hosaka M;Hanley SJ;Kudo M;Sakuragi N
通讯作者：
Sakuragi N
CAPN 7通过调节基质金属蛋白酶2活性促进人子宫内膜基质细胞迁移和侵袭
DOI：
10.1186/1477-7827-11-64
发表时间：
2013-07-15
期刊：
Reproductive biology and endocrinology : RB&E
影响因子：
--
作者：
Liu H;Jiang Y;Jin X;Zhu L;Shen X;Zhang Q;Wang B;Wang J;Hu Y;Yan G;Sun H
通讯作者：
Sun H
mmu-miR-141 在胚胎植入后早期妊娠小鼠子宫内膜中的可能作用
影响因子：
3.7
作者：
Liu X;Gao R;Chen X;Zhang H;Zheng A;Yang D;Ding Y;Wang Y;He J
通讯作者：
He J
影响因子：
4
作者：
Kessler, CA;Schroeder, JK;Handwerger, S
通讯作者：
Handwerger, S
影响因子：
13.3
作者：
Fowler, DJ;Nicolaides, KH;Miell, JP
通讯作者：
Miell, JP