Multiple novel non-canonically transcribed sub-genomic mRNAs produced by avian coronavirus infectious bronchitis virus.
Multiple novel non-canonically transcribed sub-genomic mRNAs produced by avian coronavirus infectious bronchitis virus.
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DOI：
10.1099/jgv.0.001474
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发表时间：
2020-10
期刊：
影响因子：
--
通讯作者：
Bickerton E
中科院分区：
文献类型：
--
作者：
Keep S;Oade MS;Lidzbarski-Silvestre F;Bentley K;Stevenson-Leggett P;Freimanis GL;Tennakoon C;Sanderson N;Hammond JA;Jones RC;Britton P;Bickerton E
冠状病毒亚基因组mRNA（sgmRNA）合成通过涉及互补转录调控序列（TRS）的不连续转录过程发生，其中一个（TRS-L）包含5′非翻译区（UTR）的前导序列，另一个位于每个结构和辅助基因（TRS-B）的上游。几种冠状病毒的ORF位于N基因和3′-UTR之间，由于缺乏典型的TRS-B，该区域先前被认为是传染性支气管炎病毒（IBV）中的非编码区域。在此，我们鉴定了一种非典型的TRS-B，其允许在IBV的几种毒株中产生与该ORF相关的新型sgmRNA：Beaudette、CR 88、H120、D1466、意大利-02和QX。有趣的是，由该ORF产生的潜在蛋白质在Beaudette菌株中被过早地截短。在Beaudette株中进行单核苷酸缺失，从而允许产生具有表达全长蛋白的潜力的重组IBV（rIBV）。在体外对该rIBV的评估表明，全长潜在蛋白的恢复对病毒复制没有影响。Beaudette衍生的RNA的进一步评估鉴定了位于基因2内的第二个非规范转录的sgmRNA。来自Beaudette感染的含胚卵的尿囊液的深度测序分析证实了新鉴定的非规范转录的sgmRNAs的存在，并强调了进一步未鉴定的sgmRNAs的可能性。这种HiSeq数据，以及非规范转录的sgmRNAs的确认，表明冠状病毒基因组编码比目前已确定的更大的基因库的潜力。
Coronavirus sub-genomic mRNA (sgmRNA) synthesis occurs via a process of discontinuous transcription involving complementary transcription regulatory sequences (TRSs), one (TRS-L) encompassing the leader sequence of the 5′ untranslated region (UTR), and the other upstream of each structural and accessory gene (TRS-B). Several coronaviruses have an ORF located between the N gene and the 3′-UTR, an area previously thought to be non-coding in the Gammacoronavirus infectious bronchitis virus (IBV) due to a lack of a canonical TRS-B. Here, we identify a non-canonical TRS-B allowing for a novel sgmRNA relating to this ORF to be produced in several strains of IBV: Beaudette, CR88, H120, D1466, Italy-02 and QX. Interestingly, the potential protein produced by this ORF is prematurely truncated in the Beaudette strain. A single nucleotide deletion was made in the Beaudette strain allowing for the generation of a recombinant IBV (rIBV) that had the potential to express a full-length protein. Assessment of this rIBV in vitro demonstrated that restoration of the full-length potential protein had no effect on viral replication. Further assessment of the Beaudette-derived RNA identified a second non-canonically transcribed sgmRNA located within gene 2. Deep sequencing analysis of allantoic fluid from Beaudette-infected embryonated eggs confirmed the presence of both the newly identified non-canonically transcribed sgmRNAs and highlighted the potential for further yet unidentified sgmRNAs. This HiSeq data, alongside the confirmation of non-canonically transcribed sgmRNAs, indicates the potential of the coronavirus genome to encode a larger repertoire of genes than has currently been identified.
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影响因子：
3.7
作者：
Bentley K;Armesto M;Britton P
通讯作者：
Britton P
影响因子：
2.8
作者：
Geerligs, H. J.;Boelm, G. -J.;Kumar, M.
通讯作者：
Kumar, M.
影响因子：
3.3
作者：
Dedeurwaerder A;Desmarets LM;Olyslaegers DAJ;Vermeulen BL;Dewerchin HL;Nauwynck HJ
通讯作者：
Nauwynck HJ
影响因子：
2.8
作者：
Cook, JKA;Orbell, SJ;Huggins, MB
通讯作者：
Huggins, MB
DOI：
10.1099/jgv.0.000338
发表时间：
2016-01
期刊：
The Journal of general virology
影响因子：
--
作者：
Brown PA;Touzain F;Briand FX;Gouilh AM;Courtillon C;Allée C;Lemaitre E;De Boisséson C;Blanchard Y;Eterradossi N
通讯作者：
Eterradossi N