Imaging cellulose synthase motility during primary cell wall synthesis in the grass Brachypodium distachyon.
Imaging cellulose synthase motility during primary cell wall synthesis in the grass Brachypodium distachyon.
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DOI：
10.1038/s41598-017-14988-4
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发表时间：
2017-11-08
影响因子：
4.6
通讯作者：
Baskin TI
中科院分区：
文献类型：
--
作者：
Liu D;Zehfroosh N;Hancock BL;Hines K;Fang W;Kilfoil M;Learned-Miller E;Sanguinet KA;Goldner LS;Baskin TI
纤维素合成的机制已被研究，通过表征与荧光蛋白标记的纤维素合成酶复合物的运动性，然而，这种方法已被专门用于下胚轴的拟南芥。在这里，我们的特点纤维素合酶的运动模式草，二穗短柄草。我们产生了其中mEGFP与BdCESA 3或BdCESA 6的N-末端融合的细胞系，其从野生型（Bd 21 -3）不可阻挡地生长，并且在质膜处或附近具有密集的荧光点。用粒子跟踪算法测量，GFP-BdCESA 3粒子在中胚轴中的平均速度为164 ± 78 nm min-1（误差给出标准差[SD]，n = 1451个粒子）。根部的平均速度相似。相比之下，A。表达GFP-AtCESA 6的拟南芥下胚轴的平均光吸收强度为184 ± 86 nm min-1（n = 2755）。为了B。二穗草中，我们定量根直径和伸长速率响应于纤维素（二氯苯腈; DCB），微管（安磺灵），或肌动蛋白（latrunculin B）的抑制剂。无论是安磺灵还是拉曲库林都不影响CESA复合物的速度;而DCB使B中的平均速度降低约50%。二穗穗型，A. thaliana.显然，在这些物种之间，CESA运动性很好地保守。
The mechanism of cellulose synthesis has been studied by characterizing the motility of cellulose synthase complexes tagged with a fluorescent protein; however, this approach has been used exclusively on the hypocotyl of Arabidopsis thaliana. Here we characterize cellulose synthase motility in the model grass, Brachypodium distachyon. We generated lines in which mEGFP is fused N-terminal to BdCESA3 or BdCESA6 and which grew indistinguishably from the wild type (Bd21-3) and had dense fluorescent puncta at or near the plasma membrane. Measured with a particle tracking algorithm, the average speed of GFP-BdCESA3 particles in the mesocotyl was 164 ± 78 nm min−1 (error gives standard deviation [SD], n = 1451 particles). Mean speed in the root appeared similar. For comparison, average speed in the A. thaliana hypocotyl expressing GFP-AtCESA6 was 184 ± 86 nm min−1 (n = 2755). For B. distachyon, we quantified root diameter and elongation rate in response to inhibitors of cellulose (dichlorobenylnitrile; DCB), microtubules (oryzalin), or actin (latrunculin B). Neither oryzalin nor latrunculin affected the speed of CESA complexes; whereas, DCB reduced average speed by about 50% in B. distachyon and by about 35% in A. thaliana. Evidently, between these species, CESA motility is well conserved.
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DOI：
10.1073/pnas.0706569104
发表时间：
2007-09-25
影响因子：
11.1
作者：
Desprez, Thierry;Juraniec, Michal;Vernhettes, Samantha
通讯作者：
Vernhettes, Samantha
影响因子：
4.3
作者：
BASKIN, TI;BIVENS, NJ
通讯作者：
BIVENS, NJ
DOI：
10.1073/pnas.1200352109
发表时间：
2012-03-13
影响因子：
11.1
作者：
Harris, Darby M.;Corbin, Kendall;DeBolt, Seth
通讯作者：
DeBolt, Seth
影响因子：
5.3
作者：
Handakumbura PP;Matos DA;Osmont KS;Harrington MJ;Heo K;Kafle K;Kim SH;Baskin TI;Hazen SP
通讯作者：
Hazen SP
影响因子：
6.9
作者：
Bischoff V;Cookson SJ;Wu S;Scheible WR
通讯作者：
Scheible WR