Can periodontal pockets and caries lesions act as reservoirs for coronavirus?
Can periodontal pockets and caries lesions act as reservoirs for coronavirus?
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DOI：
10.1111/omi.12362
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发表时间：
2022-04
影响因子：
3.7
通讯作者：
Fletcher, Hansel M.
中科院分区：
文献类型：
--
作者：
Natto, Zuhair S.;Afeef, Marwah;Bakhrebah, Muhammed A.;Ashi, Heba;Alzahrani, Khaled A.;Alhetheel, Abdulkarim F.;Fletcher, Hansel M.
牙周袋和可能的龋齿病变可作为全身感染传播和发展的储存库和来源。虽然牙周袋已被发现窝藏几种病毒，但没有关于其作为严重急性呼吸综合征冠状病毒2（SARS-CoV-2）储存库的能力的信息。我们使用实时聚合酶链反应（RT-PCR）方法，在一项横断面研究中评估了72名参与者中牙周袋和龋齿病变中的SARS-CoV-2，这些参与者被分为6组：牙周袋症状性COVID-19阳性病例，龋齿病变症状性阳性病例，牙周袋症状性阳性病例，龋齿病变症状性阳性病例，阳性对照和阴性对照。用RT-PCR方法扩增SARS-CoV-2 E和S基因。41.7%的牙周袋症状性COVID-19阳性病例和16.7%的空洞性龋损症状性阳性病例中存在SARS-CoV-2。牙周袋患者E和S基因的平均Ct值分别为36.06±0.46和30.06±6.73，龋病患者E和S基因的平均Ct值分别为35.73±4.14和34.78±1.93。牙周袋SARS-CoV-2检测的敏感性、特异性和准确性分别为20.8%（95%CI 7.13-42.15）、100%（95%CI 73.54-100.0）和47.2%（95%CI 30.22-64.51）。在龋蚀患者中，分别为8.3%（95%CI 1.03-27.0）、100%（95%CI 73.54-100.0）和38.9%（95%CI 23.14-56.54）。SARS-CoV-2可以在牙周袋和龋齿病变中检测到，这些部位可能是病毒的储存库。然而，与其他方法相比，SARS-CoV-2检测的灵敏度较低。据我们所知，这份报告是第一次调查SARS-CoV-2与牙周袋和龋齿之间的关系。
The periodontal pocket and likely caries lesions may act as a reservoir and source of dissemination and development of systemic infections. While periodontal pockets have been found to harbor several viral species, there is no information on its ability to serve as a reservoir for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We have used a real-time polymerase chain reaction (RT-PCR) approach to evaluate SARS-CoV-2 in periodontal pockets and cavitated caries lesions in a cross-sectional study of 72 participants who were divided into six groups: symptomatic positive COVID-19 cases with periodontal pockets, symptomatic positive with cavitated caries lesions, asymptomatic positive with periodontal pockets, asymptomatic positive with cavitated caries lesions, positive control, and negative control. A total of 180 samples were interrogated by RT-PCR to amplify the SARS-CoV-2 E and S genes. SARS-CoV-2 was present in 41.7% of symptomatic positive COVID-19 cases with periodontal pockets and 16.7% of symptomatic positive with cavitated caries lesions. The mean Ct value of E and S genes in periodontal pockets patients were 36.06±0.46 and 30.06±6.73, respectively, and the mean Ct value for both genes in caries lesions patients were 35.73±4.14, and 34.78±1.93, respectively. The sensitivity, specificity, and accuracy to detect SARS-CoV-2 among periodontal pockets were 20.8% (95% CI 7.13–42.15), 100% (95% CI 73.54–100.0), and 47.2% (95% CI 30.22–64.51), respectively. Among cavitated caries lesions patients, they were 8.3% (95% CI 1.03–27.0), 100% (95% CI 73.54–100.0), and 38.9% (95% CI 23.14–56.54), respectively. SARS-CoV-2 can be detected in periodontal pockets and caries lesions, and these sites may act as reservoirs for the virus. However, the sensitivity of SARS-CoV-2 detection is low compared with other methods. To our knowledge, this report is the first to investigate the relationship between SARS-CoV-2 and periodontal pockets and caries.
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发表时间：
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期刊：
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影响因子：
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