Expression Changes of NMDA and AMPA Receptor Subunits in the Hippocampus in rats with Diabetes Induced by Streptozotocin Coupled with Memory Impairment
Expression Changes of NMDA and AMPA Receptor Subunits in the Hippocampus in rats with Diabetes Induced by Streptozotocin Coupled with Memory Impairment
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链脲佐菌素致糖尿病合并记忆障碍大鼠海马NMDA和AMPA受体亚基的表达变化
DOI：
10.1007/s11064-019-02733-4
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发表时间：
2019-02
影响因子：
4.4
通讯作者：
Zou Ying-Ying
中科院分区：
文献类型：
--
作者：
Wang Xiao-Peng;Ye Pin;Lv Jiao;Zhou Lei;Qian Zhong-Yi;Huang Yong-Jie;Mu Zhi-Hao;Wang Xie;Liu Xin-jie;Wan Qi;Yang Zhi-Hong;Wang Fang;Zou Ying-Ying
糖尿病认知功能障碍（CID）是糖尿病（DM）的严重慢性并发症。糖尿病可能是由于N-甲基-d-天冬氨酸受体（NMDAR）和α-氨基-3-羟基-5-甲基-4-异恶唑丙酸受体（AMPAR）介导的兴奋性神经递质表达改变而导致认知功能障碍，但其发病机制尚未完全阐明，尤其是在糖尿病早期。在这里，我们试图确定认知变化，并旨在将其与大鼠海马中谷氨酸信号通路的NMDAR和AMPAR的表达变化从DM的早期阶段和在疾病进展的过程中相关联。Western blot分析和免疫荧光标记显示，糖尿病大鼠海马NMDAR亚基NR 1、NR 2A和NR 2B以及AMPAR亚基GluR 1蛋白表达明显增加。沿着，Morris水迷宫行为测试显示，与对照组相比，其表现显著下降。提示NR 1、NR 2A、NR 2B和GluR 1参与了糖尿病大鼠的学习记忆过程，其表达变化可能与糖尿病CID的发生、发展有关。
Cognitive impairment in diabetes (CID) is a severe chronic complication of diabetes mellitus (DM). It has been hypothesized that diabetes can lead to cognitive dysfunction due to expression changes of excitatory neurotransmission mediated byN-methyl-d-aspartate receptors (NMDAR) and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR); however, the pathogenesis involved in this has not been fully understood, especially at early phase of DM. Here, we sought to determine the cognitive changes and aim to correlate this with the expression changes of NMDAR and AMPAR of glutamate signaling pathways in the rat hippocampus from early phase of DM and in the course of the disease progression. By Western blot analysis and immunofluorescence labeling, the hippocampus in diabetic rats showed a significant increase in protein expression NMDAR subunits NR1, NR2A and NR2B and AMPAR subunit GluR1. Along with this, behavioral test by Morris water maze showed a significant decline in their performance when compared with the control rats. It is suggested that NR1, NR2A, NR2B and GluR1are involved in learning and memory and that their expression alterations maybe correlated with the occurrence and development of CID in diabetic rats induced by streptozotocin.
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DOI：
10.1042/cs20160397
发表时间：
2016-10-01
期刊：
Clinical science (London, England : 1979)
影响因子：
--
作者：
Hardigan T;Ward R;Ergul A
通讯作者：
Ergul A
糖尿病和认知障碍。
影响因子：
4.2
作者：
Zilliox LA;Chadrasekaran K;Kwan JY;Russell JW
通讯作者：
Russell JW
影响因子：
5.3
作者：
Fitzjohn S;Bashir Z;Farrow P
通讯作者：
Farrow P
影响因子：
--
作者：
Beckhauser TF;Francis-Oliveira J;De Pasquale R
通讯作者：
De Pasquale R
DOI：
--
发表时间：
2012
期刊：
Chinese Journal of Pathophysiology
影响因子：
--
作者：
Shang Ya-zhen
通讯作者：
Shang Ya-zhen