Courtship Conditioning/Suppression Assays in Drosophila.

Courtship Conditioning/Suppression Assays in Drosophila.
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果蝇的求爱条件/抑制测定。

DOI:
10.1101/pdb.prot108106
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发表时间:
2023
影响因子:
--
通讯作者:
Von Philipsborn AC
Von Philipsborn AC
中科院分区:
--
文献类型:
--
作者:
Von Philipsborn AC

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天真的雄性同时追求处女和交配过的雌性,但通过经验学会区分它们,从而最大限度地减少对不接受的雌性苍蝇的徒劳投资。在实验室中,我们可以利用雄性与生俱来的求偶热情,并通过将它们与非接受性雌性(未成熟的处女雌性,斩首的成熟处女雌性或成熟的交配雌性)放在一起来操纵它们的行为,称为求偶抑制/条件反射试验。早期的研究表明,雄性果蝇在与非接受性的先前交配过的雌性果蝇互动时失败,表现出求爱的动机降低(求爱抑制)。求偶抑制是研究调节短期和长期记忆的基因和神经元回路的重要实验范式。抗催情剂雄性特异性信息素11-顺式-乙烯基乙酸酯在这种条件反应中起着关键作用,因为雄性果蝇学会将其在交配雌性身上的存在与交配失败联系起来。
Naive males court both virgin and mated females but learn through experience to discriminate between them, thus minimizing futile investments in nonreceptive female flies. In the laboratory, we can exploit the innate courtship enthusiasm of males and manipulate their behavior by placing them with a nonreceptive female (immature virgin females, decapitated mature virgin females, or mature mated females), termed as the courtship suppression/conditioning assay. Early studies showed that male flies that experience failure to mate upon interaction with nonreceptive previously mated females show decreased motivation to court (courtship suppression). Courtship suppression is an important experimental paradigm for studying genes and neuronal circuits that mediate short-and long-term memory. The anti-aphrodisiac male-specific pheromone 11-cis-vaccenyl-acetate plays a key role in this conditioned response, as male flies learn to associate its presence on mated females with the failure to mate.
果蝇条件求爱:测试记忆的两种方法。
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